HeLa#18 rho-0 Cell Line
HeLa#18 rho-0 Cell Line is a mt-DNA lacking cell derived from the parental HeLa cervical carcinoma line by prolonged treatment with an inhibitor of mtDNA replication.
Highlights:
- Lacks mitochondrial DNA (rho-θ, ρ)
- Can be used as a recipient of mtDNA
- May be useful for studies related to the oxidative phosphorylation system or the effects of different mtDNA mutations (as recipient mtDNA)
From the laboratory of Mikhail F. Alexeyev, PhD, University of South Alabama.
HeLa#18 rho-0 Cell Line is a mt-DNA lacking cell derived from the parental HeLa cervical carcinoma line by prolonged treatment with an inhibitor of mtDNA replication.
Highlights:
- Lacks mitochondrial DNA (rho-θ, ρ)
- Can be used as a recipient of mtDNA
- May be useful for studies related to the oxidative phosphorylation system or the effects of different mtDNA mutations (as recipient mtDNA)
From the laboratory of Mikhail F. Alexeyev, PhD, University of South Alabama.
Specifications
| Product Type: | Cell Line |
| Name: | HeLa#18 rho-0 |
| Cell Type: | Epithelial |
| Morphology: | Epithelial |
| Source: | Cervix |
| Organism: | Human |
| Species: | Homo sapiens |
| Biosafety Level: | BSL-2 |
| Growth Conditions: | DMEM with 4.5 g/L glucose (high glucose), 50 ug/ml uridine, 1 mM sodium pyruvate, 10% fetal bovine serum, 50 ug/ml gentamycin |
| Subculturing: | 1-2 times a week. Split ratios 1:3-1:10 are tolerated well. |
| Cryopreservation: | 45% growth media, 45% heat-inactivated newborn calf serum, 10% DMSO |
| Storage: | LN2 |
| Shipped: | Dry Ice |
Provider
From the laboratory of Mikhail F. Alexeyev, PhD, University of South Alabama.
References
- Kozhukhar N, Alexeyev MF. Efficient Elimination of mtDNA from Mammalian Cells with 2',3'-Dideoxycytidine. DNA (Basel). 2024 Sep;4(3):201-211. doi: 10.3390/dna4030013. Epub 2024 Jul 4. PMID: 39035221; PMCID: PMC11259038.
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