SHSY5Y#15 rho-0 Cell Line
SHSY5Y#15 rho-0 Cell Line is a mt-DNA lacking cell derived from the parental SH-SY5Y neuroblastoma line by prolonged treatment with an inhibitor of mtDNA replication.
Highlights:
- Lacks mitochondrial DNA (rho-θ, ρ)
- Can be used as a recipient of mtDNA
- May be useful for studies related to the oxidative phosphorylation system or the effects of different mtDNA mutations (as recipient mtDNA)
From the laboratory of Mikhail F. Alexeyev, PhD, University of South Alabama.
SHSY5Y#15 rho-0 Cell Line is a mt-DNA lacking cell derived from the parental SH-SY5Y neuroblastoma line by prolonged treatment with an inhibitor of mtDNA replication.
Highlights:
- Lacks mitochondrial DNA (rho-θ, ρ)
- Can be used as a recipient of mtDNA
- May be useful for studies related to the oxidative phosphorylation system or the effects of different mtDNA mutations (as recipient mtDNA)
From the laboratory of Mikhail F. Alexeyev, PhD, University of South Alabama.
Specifications
| Product Type: | Cell Line |
| Name: | SHSY5Y#15 rho-0 |
| Cell Type: | Neuronal |
| Morphology: | Epithelial |
| Source: | Neuroblastoma. Isolated from bone marrow metastasis |
| Organism: | Human |
| Species: | Homo sapiens |
| Biosafety Level: | BSL-2 |
| Growth Conditions: | DMEM with 4.5 g/L glucose (high glucose), 50 ug/ml uridine, 1 mM sodium pyruvate, 10% fetal bovine serum, 50 ug/ml gentamycin |
| Subculturing: | Once a week. Split ratios 1:3-1:10 are tolerated well. |
| Cryopreservation: | 45% growth media, 45% heat-inactivated newborn calf serum, 10% DMSO |
| Storage: | LN2 |
| Shipped: | Dry Ice |
Provider
From the laboratory of Mikhail F. Alexeyev, PhD, University of South Alabama.
References
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