L6-GLUT4HA Rat Myoblast Cell Line
L6-GLUT4HA is an immortalized cell line derived from rat skeletal (L6) myoblast cells, and expresses the human GLUT4 with HA-epitope.
Highlights:
- Useful for studying GLUT4 levels and translocation, inflammation (sterile inflammation), insulin resistance, obesity, or type II diabetes
- Expresses the human GLUT4
- Spontaneously differentiate to myotubes upon confluence
From the laboratory of Amira Klip, PhD, Hospital For Sick Children.
L6-GLUT4HA is an immortalized cell line derived from rat skeletal (L6) myoblast cells, and expresses the human GLUT4 with HA-epitope.
Highlights:
- Useful for studying GLUT4 levels and translocation, inflammation (sterile inflammation), insulin resistance, obesity, or type II diabetes
- Expresses the human GLUT4
- Spontaneously differentiate to myotubes upon confluence
From the laboratory of Amira Klip, PhD, Hospital For Sick Children.
Specifications
| Product Type: | Cell Line |
| Name: | L6GLUT4-HA |
| Cell Type: | Myoblast |
| Morphology: | Adherent, single-nucleated cuboidal cells as myoblasts, but form multinucleated elongated and broad myotubes upon differentiation. |
| Organism: | Rat |
| Source: | 2-day neonatal rat quadricep |
| Biosafety Level: | BSL-2 |
| Growth Conditions: | Alpha-MEM, 10% FBS, antibiotic-antimycotic |
| Subculturing: |
Dissociate using Trypsin-EDTA. Doubling time 18-24h. Will spontaneously differentiate upon confluence and this can be accelerated by lowering FBS to 2% and maintaining in Alpha-MEM. |
| Cryopreservation: | Alpha-MEM, 10% FBS, 10% DMSO |
| Comments: | Culturing with 2ug/ml blasticidin-HCl maintains selective pressure for stable GLUT4-HA expression |
| Storage: | LN2 |
| Shipped: | Dry Ice |
Provider
From the laboratory of Amira Klip, PhD, Hospital For Sick Children.
Comments
The human GLUT4 cDNA chimera with a HA-epitope coding sequence inserted between the codons 67 and 68 and coincides with the first exofacial loop of the protein was provided as a gift (PMID: 8202531 ; DOI: 10.1073/pnas.91.12.5587). The GLUT4-HA cDNA was subcloned into pCXN2 and co-transfected with pSV2-bcr (blasticidin resistance) to select stable single cell clones using 5ug/ml blasticidin-HCl (PMID: 20926588; DOI: 10.1210/en.2010-0437).
References
- Tamrakar AK, Schertzer JD, Chiu TT, Foley KP, Bilan PJ, Philpott DJ, Klip A. NOD2 activation induces muscle cell-autonomous innate immune responses and insulin resistance. Endocrinology. 2010 Dec;151(12):5624-37. doi: 10.1210/en.2010-0437. Epub 2010 Oct 6. PMID: 20926588.
- Antonescu CN, Ishikura S, Bilan PJ, Klip A. Measurement of GLUT4 Traffic to and from the Cell Surface in Muscle Cells. Curr Protoc. 2023 Jun;3(6):e803. doi: 10.1002/cpz1.803. PMID: 37367531.
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