L6-GLUT4myc-IR Rat Myoblast Cell Line
L6-GLUT4myc-IR is an immortalized cell line derived from rat skeletal (L6) myoblast cells, and expresses the human GLUT4 with a myc-epitope and the human insulin receptor (IR).
Highlights:
- Useful for studying inflammation (sterile inflammation), insulin resistance, obesity, or type II diabetes
- Expresses the human insluin receptor (IR)
- Highly sensitive insulin-stimulated GLUT4myc translocation
- Amenable to generation of insulin resistance and screens of insulin sensitizing compounds
From the laboratory of Amira Klip, PhD, Hospital For Sick Children.
L6-GLUT4myc-IR is an immortalized cell line derived from rat skeletal (L6) myoblast cells, and expresses the human GLUT4 with a myc-epitope and the human insulin receptor (IR).
Highlights:
- Useful for studying inflammation (sterile inflammation), insulin resistance, obesity, or type II diabetes
- Expresses the human insluin receptor (IR)
- Highly sensitive insulin-stimulated GLUT4myc translocation
- Amenable to generation of insulin resistance and screens of insulin sensitizing compounds
From the laboratory of Amira Klip, PhD, Hospital For Sick Children.
Specifications
| Product Type: | Cell Line |
| Name: | L6GLUT4myc-IR |
| Cell Type: | Myoblast |
| Morphology: | Adherent, single-nucleated cuboidal cells as myoblasts, but form multinucleated elongated and broad myotubes upon differentiation. |
| Organism: | Rat |
| Source: | 2-day neonatal rat quadricep |
| Biosafety Level: | BSL-2 |
| Growth Conditions: | Alpha-MEM, 10% FBS, antibiotic-antimycotic |
| Subculturing: |
Doubling time 18-24h; Use trypsin to dissociate the cells. Will spontaneously differentiate upon confluence and this can be accelerated by lowering FBS to 2% and maintaining in Alpha-MEM. |
| Cryopreservation: | Alpha-MEM, 10% FBS, 10% DMSO |
| Comments: | Culturing with 2ug/ml puromycin maintains selective pressure for stable Insulin Receptor protein expression. |
| Storage: | LN2 |
| Shipped: | Dry Ice |
Provider
From the laboratory of Amira Klip, PhD, Hospital For Sick Children.
Comments
The human Insulin Receptor protein (IR) cDNA (PMID: 2859121 DOI: 10.1016/0092-8674(85)90334-4) was subcloned into the retroviral vector pBABE-puro and transfected into ecotropic Phoenix packaging cells to prepare retroviral particles. L6-GLUT4myc myoblasts (PMID: 9607310 DOI: 10.1016/s0014-5793(98)00423-2) were infected with these retroviruses and a stable pool of cells were selected in growth medium containing 2ug/ml of puromycin and immunoblotting confirmed overexpression of the IR protein (PMID: 20926588; DOI: 10.1210/en.2010-0437).
References
- Tamrakar AK, Schertzer JD, Chiu TT, Foley KP, Bilan PJ, Philpott DJ, Klip A. NOD2 activation induces muscle cell-autonomous innate immune responses and insulin resistance. Endocrinology. 2010 Dec;151(12):5624-37. doi: 10.1210/en.2010-0437. Epub 2010 Oct 6. PMID: 20926588.
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