C2C12-GLUT4HA Rat Myoblast Cell Line
C2C12-GLUT4HA is an immortalized cell line derived from the mouse C2C12 myoblast cells, and expresses the human GLUT4 with HA-epitope.
Highlights:
- Useful for studying GLUT4 levels and translocation, inflammation (sterile inflammation), insulin resistance, obesity, or type II diabetes
- Expresses the human GLUT4
- Spontaneously differentiate to myotubes upon confluence
From the laboratory of Amira Klip, PhD, Hospital For Sick Children.
C2C12-GLUT4HA is an immortalized cell line derived from the mouse C2C12 myoblast cells, and expresses the human GLUT4 with HA-epitope.
Highlights:
- Useful for studying GLUT4 levels and translocation, inflammation (sterile inflammation), insulin resistance, obesity, or type II diabetes
- Expresses the human GLUT4
- Spontaneously differentiate to myotubes upon confluence
From the laboratory of Amira Klip, PhD, Hospital For Sick Children.
Specifications
| Product Type: | Cell Line |
| Name: | C2C12GLUT4-HA |
| Cell Type: | Myoblast |
| Morphology: | Adherent, single-nucleated cuboidal cells as myoblasts, but form multinucleated elongated myotubes upon differentiation. |
| Organism: | Mouse |
| Source: | 2-day regenerating adult quadricep (from C3H mouse) |
| Biosafety Level: | BSL-2 |
| Growth Conditions: | DMEM (4.5g/L glucose), 2 mM glutamine, 10% FBS, antibiotic-antimycotic |
| Subculturing: |
Dissociate using Trypsin-EDTA. Doubling time 18-24h. They will spontaneously differentiate upon confluence and this can be accelerated by switching to 5% Horse serum or 2% FBS while maintaining in DMEM. |
| Cryopreservation: | DMEM, 20% FBS, 10% DMSO |
| Comments: | Culturing with 5ug/ml blasticidin-HCl maintains selective pressure for stable GLUT4-HA expression |
| Storage: | LN2 |
| Shipped: | Dry Ice |
Provider
From the laboratory of Amira Klip, PhD, Hospital For Sick Children.
Comments
The human GLUT4 cDNA chimera with a HA-epitope coding sequence inserted between the codons 67 and 68 and coincides with the first exofacial loop of the protein was provided as a gift (PMID: 8202531 ; DOI: 10.1073/pnas.91.12.5587). The GLUT4-HA cDNA was subcloned into pCXN2 and co-transfected with pSV2-bcr (blasticidin resistance) into C2C12 myoblasts (from the ATCC: http://www.atcc.org, cat. no. CRL-1772) to select stable single cell clones using 10ug/ml blasticidin-HCl. GLUT4-HA expression was confirmed. GLUT4-HA translocation responds to electrically stimulated contraction and AMPK activation in C2C12GLUT4-HA myotubes (PMID: 29089333 DOI: 10.1152/ajpendo.00103.2017).
References
- Li Z, Yue Y, Hu F, Zhang C, Ma X, Li N, Qiu L, Fu M, Chen L, Yao Z, Bilan PJ, Klip A, Niu W. Electrical pulse stimulation induces GLUT4 translocation in C2C12 myotubes that depends on Rab8A, Rab13, and Rab14. Am J Physiol Endocrinol Metab. 2018 May 1;314(5):E478-E493. doi: 10.1152/ajpendo.00103.2017. Epub 2017 Oct 31. PMID: 29089333.
- Antonescu CN, Ishikura S, Bilan PJ, Klip A. Measurement of GLUT4 Traffic to and from the Cell Surface in Muscle Cells. Curr Protoc. 2023 Jun;3(6):e803. doi: 10.1002/cpz1.803. PMID: 37367531.
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