MCF-7 rho-0#5d Cell Line
MCF-7 rho-0#5d Cell Line is a mt-DNA lacking cell derived from the parental MCF-7 cell line by prolonged treatment with an inhibitor of mtDNA replication.
Highlights:
- Lacks mitochondrial DNA (rho-θ, ρ-0)
- Can be used as a recipient of mtDNA
- May be useful for studies related to the oxidative phosphorylation system or the effects of different mtDNA mutations (as recipient mtDNA)
From the laboratory of Mikhail F. Alexeyev, PhD, University of South Alabama.
MCF-7 rho-0#5d Cell Line is a mt-DNA lacking cell derived from the parental MCF-7 cell line by prolonged treatment with an inhibitor of mtDNA replication.
Highlights:
- Lacks mitochondrial DNA (rho-θ, ρ-0)
- Can be used as a recipient of mtDNA
- May be useful for studies related to the oxidative phosphorylation system or the effects of different mtDNA mutations (as recipient mtDNA)
From the laboratory of Mikhail F. Alexeyev, PhD, University of South Alabama.
Specifications
| Product Type: | Cell Line |
| Name: | MCF-7 rho-0#5d Cell Line |
| Cell Type: | Breast epithelium |
| Accession ID: | CVCL_0031 |
| Morphology: | Epithelial |
| Organism: | Human |
| Source: | Breast |
| Biosafety Level: | BSL-2 |
| Subculturing: | Split 1:2 to 1:3 every 2-3 weeks |
| Growth Conditions: | DMEM, 20% FBS, 4.5 g/l glucose, 1 mM sodium pyruvate, 50 ug/ml uridine, 37C, 5% CO2 |
| Cryopreservation: | 45% growth medium, 45% heat-inactivated calf serum, 10% DMSO |
| Comments: | Cells grow very slowly. Population doubling time ~250-300h |
| Storage: | LN2 |
| Shipped: | Dry Ice |
Documentation
Provider
From the laboratory of Mikhail F. Alexeyev, PhD, University of South Alabama.
Comments
MCF-7 cells were treated with an inhibitor of mitochondrial DNA (mtDNA) replication for 69 days, after which surviving cells were cloned, the resulting clones were analyzed for mtDNA content by PCR, and the cells' identity was confirmed by STR analysis. Clone #5d lacks mitochondrial DNA (rho-0; ρ-0) and can be used as a recipient of mtDNA. Its STR profile matches that of Cellosaurus MCF-7 (CVCL_0031).
References
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