This IgG1 mouse mononoclonal antibody was generated against the partially purified human neutrophil flavocytochrome b (cytochrome b559 or cytochrome b558) and is reactive against human gp91phox amino acids 382-389.
Gp91phox (portion of the heterodimer Cytochrome b) is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. It also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. Defects in the CYBB gene are a cause of X-linked chronic granulomatous disease (X-CGD). X-CGD is characterized by the failure of activated phagocytes to generate superoxide and patients suffer from life-threatening bacterial/fungal infections.
From the laboratory of Algirdas J. Jesaitis, PhD, Montana State University.
Part of The Investigator's Annexe program.
Product Type: | Antibody |
Name: | Anti-NOX2/gp91phox [54.1] Antibody (Alternative names: AMCBX2 antibody, CGD antibody, CGD91-phox antibody, CY24B_HUMAN antibody, CYBB antibody) |
Accession ID: | P04839 |
Molecular Weight: | 65 kDa |
Isotype: | IgG1 |
Clonality: | Monoclonal |
Clone Name: | 54.1 |
Reactivity: | Human |
Specificity: | Human gp91phox 382-PKIAVDGP-389 |
Immunogen: | Partially purified human neutrophil flavocytochrome b (heparin Ultrogel/lectin affinity purification ? in Triton X-100) |
Species Immunized: | Mouse |
Myeloma Used: | SP2/0 |
Purification Method: | Protein G |
Buffer: | 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide |
Tested Applications: | WB (1:1000) |
Storage: | -20C |
Shipped: | Cold packs |
Specificity of mAb for cytochrome b and phage pIII proteins. Intact phage expressing known amino acid sequences (lanes B, C, E, and F) and partially purified cytochrome b (lanes A and D) were separated on SDS-PAGE polyacrylamide gels and transferred to nitrocellulose. Samples were Western blotted with mAb 54.1 (lanes A-C) or mAb 44.1 (lanes D-F). Lanes B and F contain proteins of phage sequence 3 (PQVRPI), and lanes C and E contain proteins of phage sequence 27 (FKRGVD.
Adapted from: Burritt, J.B. et al. J.Biol.Chem. 270:16974-16980, 1995.
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