Anti-NOX2/gp91phox [CL5] Antibody
![Anti-NOX2/gp91phox [CL5] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/845_200120200126248440.jpg "Anti-NOX2/gp91phox [CL5] Antibody")
This IgG1 mouse mononoclonal antibody was generated against the partially purified human neutrophil flavocytochrome b (cytochrome b559 or cytochrome b558) and is reactive against human gp91phox amino acids 382-389. Clone CL5 has also been shown to cross-reacte with cytosolic and membrane-bound gelsolin.
Gp91phox (portion of the heterodimer Cytochrome b) is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. It also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. Defects in the CYBB gene are a cause of X-linked chronic granulomatous disease (X-CGD). X-CGD is characterized by the failure of activated phagocytes to generate superoxide and patients suffer from life-threatening bacterial/fungal infections.
From the laboratory of Algirdas J. Jesaitis, PhD, Montana State University.
Part of The Investigator's Annexe program.
This IgG1 mouse mononoclonal antibody was generated against the partially purified human neutrophil flavocytochrome b (cytochrome b559 or cytochrome b558) and is reactive against human gp91phox amino acids 382-389. Clone CL5 has also been shown to cross-reacte with cytosolic and membrane-bound gelsolin.
Gp91phox (portion of the heterodimer Cytochrome b) is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. It also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. Defects in the CYBB gene are a cause of X-linked chronic granulomatous disease (X-CGD). X-CGD is characterized by the failure of activated phagocytes to generate superoxide and patients suffer from life-threatening bacterial/fungal infections.
From the laboratory of Algirdas J. Jesaitis, PhD, Montana State University.
Part of The Investigator's Annexe program.
| Product Type: | Antibody |
| Name: | Anti-NOX2/gp91phox [CL5] Antibody |
| Accession ID: | P04839 |
| Isotype: | IgG1 |
| Clonality: | Monoclonal |
| Clone Name: | CL5 |
| Specificity: | Human gp91phox ? 135-DPYSVALSELGDR-147 |
| Reactivity: | Human |
| Immunogen: | Partially purified human neutrophil flavocytochrome b (heparin Ultrogel/lectin affinity purification ? in Triton X-100) |
| Species Immunized: | Mouse |
| Myeloma Used: | P3U1 |
| Purification Method: | Protein G |
| Buffer: | PBS, 0.05% (w/v) Sodium Azide |
| Tested Applications: | WB (1:1000) |
| Storage: | -20C |
| Shipped: | Cold packs |
- Baniulis, D., Burritt, J.B., Taylor, R.M, Dinauer, M.C., Heyworth, P.G., Parkos, C.A., Magnusson, K.-E., Jesaitis, A.J. Monoclonal antibody CL5 recognizes the amino terminal domain of human phagocyte flavocytochrome b558 large subunit gp91phox. Eur. J. Hematology 74(4):337-47, 2005.
If you publish research with this product, please let us know so we can cite your paper.
