This polyphosphate (Long Chain, Biotinylated) is heterogeneous in size, with approximate polymer lengths ranging from ~200-1300 phosphate units.
Highlights
Polyphosphate, a linear polymer of inorganic orthophosphate, which is widespread in living organisms and which plays diverse roles in biology, has recently been shown to be a potent modulator of the human blood clotting system. Its procoagulant activity is dependent on polymer length; this high MW polyphosphate preparation has very high specific activity in plasma clotting assays.
In addition to being a useful tool for plasma clotting assays, polyphosphate is can be used to study bacterial growth, virulence, and bacterial membrane function.
Also available:
From the laboratory of James H. Morrissey, PhD, University of Michigan.
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Product Type: | Small Molecule |
Name: | Polyphosphate, Long Chain (Biotinylated) |
Chemical Formula: | (NaPO3)n[actual counterions may be Na &/or Li; nis approximately 900 to 1100] |
Molecular Weight: | Heterogeneous; Number average ~100 kDa; Range ~8 kDa to 192 kDa |
Format: | Lyophilized |
Purity: | <1% monophosphate |
Buffer: | 20 mM Hepes-NaOH pH 8.0, 5 mM EDTA |
Solubility: | At least 100 mM in water; <1 mM in buffers containing divalent metals (make intermediate dilutions in divalent metal ion free buffers) |
Concentration: | When reconstituted:10 mM (phosphate monomer) NOTE: To estimate the polymer concentration, divide the phosphate monomer concentration by the modal polymer length |
Size Distribution: | Mode of n: 900-1100 (as measured by migration on PAGE) |
Comments: | It is recommended to use ~10.2 ug of the biotin-polyphosphate to coat all wells of a streptavidin-coated 96-well plate. |
Storage: |
Dry material should be stored at room temperature (or lower) with dessication. Once reconstituted: - Stable at least 12 hours at room temperature - Stable at least 72 hours at 4C - Store long-term at -80C - Aliquot for repeated use, avoiding repeated freeze-thaw cycles - Do not store in the presence of divalent metal ions |
Shipped: | Ambient temperature |
Size Distribution
Polyphosphate has limited solubility in the presence of divalent metal ions, so make intermediate dilutions in water, or in buffers without divalent metals or polyphosphate-binding proteins.
Reconstitution:
1 - Centrifuge tube 1 min at 1000 x g to pellet material in bottom of tube.
2 - Reconstitute in 118 µL purified water to make a solution of 10 mM phosphate.
NOTE: May be reconstituted in larger volumes to make a more dilute solution as needed.
If you publish research with this product, please let us know so we can cite your paper.