Immortalized human epithelial cell line (A2EN; passage >50) derived from human primary epithelial cells expanded from an explant of human endocervical tissue.
Highlights:
The endocervical epithelium is a single cell layer lining the endocervical canal that separates the lower female reproductive tract from the uterus and Fallopian tubes. Endocervical epithelial cells secrete mucus and innate immune mediators that facilitate reproduction as well as protect from pathogen invasion. The endocervix is a major site of transmission for multiple sexually transmitted organisms, such as Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, and Human Immunodeficiency Virus (HIV).
From the laboratory of Alison J. Quayle, PhD, LSU Health Sciences Center.
Part of The Investigator's Annexe program.
Product Type: | Cell Line |
Name: | Human Endocervical Epithelial Cell Line (A2EN) |
Cell Type: | Epithelial |
Accession ID: | CVCL_0P36 |
Source: | Endocervix |
Organism: | Human |
Morphology: | Keratinocyte appearance when cultured on plastic; cobblestone appearance with occasional multilayer regions when grown on cell culture inserts |
Biosafety Level: | 2 |
Growth Conditions: | Epilife+EDGS or KSFM+BPE/EGF (Either medium supplemented with 0.06mM-0.4mM Calcium Chloride), 5% CO2, 37C |
Subculturing: | 1 week |
Cryopreservation: | Growth medium containing 5% FBS + 5% DMSO |
Storage: | Liquid nitrogen |
Shipped: | Dry ice |
C. trachomatis-Infected Endocervical Epithelial Cell (A2En) Culture.
The immortalized endocervical epithelial cell line A2EN was exposed to C. trachomatis serovar D at multiplicity of infection of 2 in the absence of cyclohexamide (right two panels). This resulted in a mixed population of infected and uninfected cells. Infected cells are stained green with chlamydial LPS conjugated to FITC (bottom), and nucleic acid is stained with DAPI (blue, top). Uninfected controls are included in the left two panels for comparison. Data is representative of experiments performed greater than 10 times.
Adapted from: Ibana JA., et al. Infect Dis Obstet Gynecol. 2011; 2011: 420905.
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