Mouse Macrophage Cell Line (AA9) for PRRSV Propagation
Sialoadhesin expressing macrophage cell-line [AA9] for in-vitro propagation of porcine reproductive and respiratory syndrome virus (PRRSV).
Highlights:
- Ideal cell line for the propagation of PRRSV in vitro
- Transfected with porcine sialoadhesin - allows for permissive PRRSV replication
Porcine reproductive and respiratory syndrome virus (PRRSV) also known has blue-ear pig syndrome is an small enveloped RNA virus that causes disease in pigs. The virus effects reproductive failure in breeding stock and respiratory tract illness in young pigs. Sialoadhesin is the cell-surface protein on porcine alveolar macrophages (PAMs) that mediates the internalization of porcine reproductive and respiratory syndrome virus (PRRSV).
From the laboratory of Subramaniam (Sri) Srikumaran, PhD, Washington State University.
Part of The Investigator's Annexe program.
Sialoadhesin expressing macrophage cell-line [AA9] for in-vitro propagation of porcine reproductive and respiratory syndrome virus (PRRSV).
Highlights:
- Ideal cell line for the propagation of PRRSV in vitro
- Transfected with porcine sialoadhesin - allows for permissive PRRSV replication
Porcine reproductive and respiratory syndrome virus (PRRSV) also known has blue-ear pig syndrome is an small enveloped RNA virus that causes disease in pigs. The virus effects reproductive failure in breeding stock and respiratory tract illness in young pigs. Sialoadhesin is the cell-surface protein on porcine alveolar macrophages (PAMs) that mediates the internalization of porcine reproductive and respiratory syndrome virus (PRRSV).
From the laboratory of Subramaniam (Sri) Srikumaran, PhD, Washington State University.
Part of The Investigator's Annexe program.
| Product Type: | Cell Line |
| Name: | AA9 |
| Cell Type: | Macrophage |
| Accession ID: | CVCL_0P26 |
| Organism: | Mouse |
| Source: | Ascites |
| Morphology: | Macrophage |
| Biosafety Level: | I |
| Growth Conditions: | DMEM +10% FBS +2mM Glutamine and 500ug/mL G418 |
| Subculturing: | Scraping or trypsin can be used. Every 3-4 days into 1:4 |
| Cryopreservation: | 90% FBS + 10% DMSO |
| Format: | Live cells (at 50% confluency) in a T25 flask |
| Storage: | Liquid nitrogen |
| Shipped: | Ambient temperature as live cells (at 50% confluency) in a T25 flask |
- Shanthalingam S, Liu W, Snekvik K, Pattnaik A, Osorio FA, Srikumaran S. 2009. Transfection of porcine sialoadhesin into a murine macrophage cell-line renders it permissive for PRRSV replication. Veterinary Immunology and Immunopathology. 128:240-241
- Srikumaran S., Liu W., Shanthalingam S. Macrophage cell-lines for propagation of porcine reproductive and respiratory syndrome virus. US Patent 2010/0146653 A1, June 10, 2010.
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