RyR1 Expressing HEK293 Cell Line
Immortalized cell line HEK293 that stably expresses the type 1 ryanodine receptor (RyR1).
Highlights:
- Cell line that stably expresses RyR1
- RyR1 expression validated by immunoblotting and immunocytochemistry techniques
- RyR1 function validated by calcium release in response to addition of RyR1 agonist 4-cholo-m-cresol
- RyR1 single channel activity validated in nuclear patch clamp experiments
The type I ryanodine receptor (RyR1), also known as skeletal muscle calcium release channel or skeletal muscle-type ryanodine receptor, is a protein found primarily in skeletal muscle. The main role of this protein is to form an ion channel that transports calcium ions from an intracellular sarco/endoplasmic reticulum storage compartment to the cytoplasm. Additionally, the RyR1 protein plays a critical role in muscles that are used for movement by causing them to contract.
From the laboratory of Robert T. Dirksen, PhD, University of Rochester.
Part of The Investigator's Annexe program.
Immortalized cell line HEK293 that stably expresses the type 1 ryanodine receptor (RyR1).
Highlights:
- Cell line that stably expresses RyR1
- RyR1 expression validated by immunoblotting and immunocytochemistry techniques
- RyR1 function validated by calcium release in response to addition of RyR1 agonist 4-cholo-m-cresol
- RyR1 single channel activity validated in nuclear patch clamp experiments
The type I ryanodine receptor (RyR1), also known as skeletal muscle calcium release channel or skeletal muscle-type ryanodine receptor, is a protein found primarily in skeletal muscle. The main role of this protein is to form an ion channel that transports calcium ions from an intracellular sarco/endoplasmic reticulum storage compartment to the cytoplasm. Additionally, the RyR1 protein plays a critical role in muscles that are used for movement by causing them to contract.
From the laboratory of Robert T. Dirksen, PhD, University of Rochester.
Part of The Investigator's Annexe program.
| Product Type: | Cell Line |
| Name: | HEK293-RyR1 |
| Cell Type: | HEK293 |
| Accession ID: | P21817, CVCL_0P75 |
| Morphology: | Flat/Circular |
| Source: | Kidney |
| Organism: | Human |
| Biosafety Level: | BSL2 |
| Subculturing: | Usually a 1:5 or 1:10 dilution will be 80-90% confluent in 3-4 days |
| Growth Conditions: | DMEM (High Glucose), 10% FBS, 2mM L-Glutamine, 1% Pen/Strep, 100µg/ml Hygromycin |
| Cryopreservation: | 90% complete media with 10% DMSO |
| Storage: | Liquid nitrogen |
| Shipped: | Dry ice |
Protocol notes
Remove media from flask and rinse with 1X PBS without calcium and magnesium. Add Trypsin-EDTA (0.05%) and put in 37°C incubator until cells detach. Add some fresh media to stop trypsinization (2X as much as added trypsin). Transfer some of cell suspension to a new flask containing fresh media to maintain cells.
RyR1 expression in stable HEK293-RyR1 cells
Control Flp-In 293 cells (Panel I) and HEK-RyR1 cells (Panel II) showing Hoechst 34580 nuclear staining alone (left), RyR1 immunostaining alone (middle) and overlay (right).
Adapted from: Wagner LE, et al., Cell Calcium. 2014 Aug;56(2):96-107.
- Wagner LE, Groom LA, Dirksen RT, Yule DI. Characterization of ryanodine receptor type 1 single channel activity using "on-nucleus" patch clamp. Cell Calcium. 2014 Aug;56(2):96-107.
If you publish research with this product, please let us know so we can cite your paper.
