HaloTag-RealThiol (HaloRT) Universal Organelle Specific Glutathione (GSH) Probe
The Halo-RealThiol (HaloRT) probe provides ratiometric fluorescent readout for quantification of glutathione in live mammalian cells.
Highlights:
- HaloRT is cell permeable and can measure GSH levels in any organelle of interest
- Conveniently compare organelle-specific GSH levels in live cells using confocal microscopy
- Signals from blue and green channels allow for deduction of organelle specific GSH concentration, given appropriate calibration curve
- Validated in the nucleus, cytosol, mitochondria, and endoplasmic reticulum (ER) in HeLa cells
- This item is HaloRT ligand and must be used in cells expressing HaloTag protein
Subcellular detection and localization of GSH is important in understanding the modulation of redox status, the effect of drugs, and the mechanisms of detoxification. Furthermore, differences in GSH levels in response to oxidative stress in subpopulations of cells have been reported, underscoring the importance of detection methods amenable to flow cytometry and automated fluorescence detection.
From the laboratory of Jin Wang, PhD, Baylor College of Medicine.
The Halo-RealThiol (HaloRT) probe provides ratiometric fluorescent readout for quantification of glutathione in live mammalian cells.
Highlights:
- HaloRT is cell permeable and can measure GSH levels in any organelle of interest
- Conveniently compare organelle-specific GSH levels in live cells using confocal microscopy
- Signals from blue and green channels allow for deduction of organelle specific GSH concentration, given appropriate calibration curve
- Validated in the nucleus, cytosol, mitochondria, and endoplasmic reticulum (ER) in HeLa cells
- This item is HaloRT ligand and must be used in cells expressing HaloTag protein
Subcellular detection and localization of GSH is important in understanding the modulation of redox status, the effect of drugs, and the mechanisms of detoxification. Furthermore, differences in GSH levels in response to oxidative stress in subpopulations of cells have been reported, underscoring the importance of detection methods amenable to flow cytometry and automated fluorescence detection.
From the laboratory of Jin Wang, PhD, Baylor College of Medicine.
Specifications
| Product Type: | Small Molecule |
| Name: | Halo-RealThiol (HaloRT) Ligand |
| Chemical Formula: | C31H41ClN4O6 |
| Molecular Weight: | 601.14 |
| Format: | Red to orange solid (Lyophilized powder in tubes) |
| Purity: | >97%, HPLC |
| Tested Applications: | 1 µM for cell staining |
| Solubility: | DMSO |
| Spectral Information: | ex/em: HaloRT: 488/565; HaloRT-GSH: 405/488 |
| Platform: | Confocal microscopy/FACS |
| Compatible Cells: | Mammalian cells e.g. HeLa, primary hepatocytes |
| Detection Method: | Fluorescence |
| Comments: |
- Dilute DMSO stock with imaging buffer. - A laser-based light source is preferred for best results. - This item is HaloRT ligand and must be used in cells expressing HaloTag protein. |
| Storage: | -80C |
| Shipped: | Ambient Temperature |
Documentation
Provider
From the laboratory of Jin Wang, PhD, Baylor College of Medicine.
References
- Xiqian Jiang, Chengwei Zhang, Jianwei Chen, Sungwoo Choi, Ying Zhou, Mingkun Zhao, Xianzhou Song, Xi Chen, Mirjana Maletic-Savatic, Timothy Palzkill, David Moore, Meng C. Wang, and Jin Wang*, "Quantitative Real-Time Imaging of Glutathione with Subcellular Resolution", Antioxidants & Redox Signaling.
- Jiang X, Chen J, Wang MC, Wang J. Glutathione Quantification in Live Cells with Real-Time Imaging and Flow Cytometry. STAR Protoc. 2020 Nov 14;1(3):100170. View Article
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