Anti-Human/Murine Chimeric Flavivirus (6B6C-1) Antibodies
These mouse antibodies incorporate the variable regions of broadly flavivirus cross-reactive mAb 6B6C-1 (specific for the flaviviral envelope (E) protein) into a plasmid construct containing the human IgG γ1 chain. The antibodies recognize Flavivirus (6B6C-1).
Highlights:
- 6ME2: Reacts with the E protein of the West Nile Virus (WNV)-VLP (virus like particles), Yellow Fever Virus (YFV) and Saint Louis Encephalitis Virus (SLEV) seed antigens in MAC-ELISA, Suckling Mouse Brain (SMB) and VLP antigens in MAC-ELISA
- 6GF4: Reacts with YFV, WNV and Japanese encephalitis virus (JEV); Acheives positive P/N values with each flaviviral antigen tested in the indirect IgG ELISA
- Suitable for Western Blot and ELISA applications
The flavivirus inlcudes West Nile virus, dengue virus, tick-borne encephalitis virus, yellow fever virus, Zika virus and several other viruses which may cause encephalitis. Flaviviruses have a (+) sense RNA genome and replicate in the cytoplasm of the host cells. The genome mimics the cellular mRNA molecule in all aspects except for the absence of the poly-adenylated (poly-A) tail. This feature allows the virus to exploit cellular apparatus to synthesise both structural and non-structural proteins, during replication.
From the laboratory of Centers for Disease Control and Prevention.
These mouse antibodies incorporate the variable regions of broadly flavivirus cross-reactive mAb 6B6C-1 (specific for the flaviviral envelope (E) protein) into a plasmid construct containing the human IgG γ1 chain. The antibodies recognize Flavivirus (6B6C-1).
Highlights:
- 6ME2: Reacts with the E protein of the West Nile Virus (WNV)-VLP (virus like particles), Yellow Fever Virus (YFV) and Saint Louis Encephalitis Virus (SLEV) seed antigens in MAC-ELISA, Suckling Mouse Brain (SMB) and VLP antigens in MAC-ELISA
- 6GF4: Reacts with YFV, WNV and Japanese encephalitis virus (JEV); Acheives positive P/N values with each flaviviral antigen tested in the indirect IgG ELISA
- Suitable for Western Blot and ELISA applications
The flavivirus inlcudes West Nile virus, dengue virus, tick-borne encephalitis virus, yellow fever virus, Zika virus and several other viruses which may cause encephalitis. Flaviviruses have a (+) sense RNA genome and replicate in the cytoplasm of the host cells. The genome mimics the cellular mRNA molecule in all aspects except for the absence of the poly-adenylated (poly-A) tail. This feature allows the virus to exploit cellular apparatus to synthesise both structural and non-structural proteins, during replication.
From the laboratory of Centers for Disease Control and Prevention.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
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| Product Type: | Antibody |
| Antigen: | Flaviviral envelope (E) protein |
| Isotype: |
6GF4: IgG1 6ME2: IgM |
| Clonality: | Monoclonal |
| Clone Name: |
6GF4 6ME2 |
| Immunogen: |
6GF4: pdHL2-6G, VH and VK regions of mMAb 6B6C-1 was incorporated separately into the human IgG expression construct pdHL2 generating plasmid pdHL2-6G (6B6C-1:IgG) 6ME2: The VH and VK regions of 6B6C-1 were incorporated into the human IgM expression construct pJH2 by ligation, generating plasmid pJH-6M (6B6C-1:IgM) |
| Species Immunized: | None; Sp2/0-Ag14 (Sp2) murine myeloma cells |
| Buffer: | Cell Culture Supernatant |
| Storage: | -80C |
| Shipped: | Cold Packs |
