Yeast-Display Nanobody Library (NbLib)
Yeast surface display nanobody library (NbLib) for rapid discovery of conformationally selective nanobodies.
Highlights
- Fully in vitro platform for nanobody discovery based on yeast surface display
Camelid single-domain antibody fragments (‘nanobodies’) provide the remarkable specificity of antibodies within a single 15-kDa immunoglobulin VHH domain. This unique feature has enabled applications ranging from use as biochemical tools to therapeutic agents. Nanobodies have emerged as especially useful tools in protein structural biology, facilitating studies of conformationally dynamic proteins such as G-protein-coupled receptors (GPCRs).
From the laboratory of Andrew C. Kruse, PhD, Harvard University.
Yeast surface display nanobody library (NbLib) for rapid discovery of conformationally selective nanobodies.
Highlights
- Fully in vitro platform for nanobody discovery based on yeast surface display
Camelid single-domain antibody fragments (‘nanobodies’) provide the remarkable specificity of antibodies within a single 15-kDa immunoglobulin VHH domain. This unique feature has enabled applications ranging from use as biochemical tools to therapeutic agents. Nanobodies have emerged as especially useful tools in protein structural biology, facilitating studies of conformationally dynamic proteins such as G-protein-coupled receptors (GPCRs).
From the laboratory of Andrew C. Kruse, PhD, Harvard University.
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| Product Type: | Bacteria |
| Name: | Yeast-Display Nanobody Library (NbLib) |
| Organism: | S. cerevisiae |
| Strain: | BJ5465 |
| Growth Conditions: |
Yglc4.5 –Trp media, shake at 230 RPM, 30C.  Suggested Protocol
|
| Cryopreservation: | Yglc4.5 –Trp media + 10% DMSO |
| Comments: | Includes two vials of library and one vial of sample cells for users to perform trial runs of growing yeast cultures, should they wish. |
| Storage: | -80C |
| Shipped: | Dry ice |
- McMahon C, Baier AS, Pascolutti R, Wegrecki M, Zheng S, Ong JX, Erlandson SC, Hilger D, Rasmussen SGF, Ring AM, Manglik A, Kruse AC. Yeast surface display platform for rapid discovery of conformationally selective nanobodies. Nat Struct Mol Biol. 2018 Mar;25(3):289-296. doi: 10.1038/s41594-018-0028-6. Epub 2018 Feb 12.
- Hoey RJ, Eom H, Horn JR. Structure and development of single domain antibodies as modules for therapeutics and diagnostics. Exp Biol Med (Maywood). 2019 Dec;244(17):1568-1576. View article
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