4B6 Cell Line
This cell line is a derivative of the Cre4 cell line (Cat. ESA109). Cre4 cells were engineered for Tet-On expression with a retrovirus 2641 (Mol. Biol. Rep. 37 (2010) 1987-1991), which encodes a Tet-On Advanced transactivator, EGFP, and blasticidin resistance, thus producing a clone designated 4B6.
From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.
This cell line is a derivative of the Cre4 cell line (Cat. ESA109). Cre4 cells were engineered for Tet-On expression with a retrovirus 2641 (Mol. Biol. Rep. 37 (2010) 1987-1991), which encodes a Tet-On Advanced transactivator, EGFP, and blasticidin resistance, thus producing a clone designated 4B6.
From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.
Specifications
| Product Type: | Cell Line |
| Cell Type: | Embryonic fibroblasts |
| Source: | Mouse E13.5 Embryo |
| Organism: | Mouse |
| Morphology: | Spindle-shaped at confluence |
| Biosafety Level: | BSL 1 |
| Subculturing: | Split 1:4 to 1:8 every 3 days |
| Growth Conditions: | DMEM/10% FBS+10 µg/ml blasticidin |
| Cryopreservation: | Media + 10% DMSO |
| Comments: | Fast Growing |
| Storage: | LN2 |
| Shipped: | Dry Ice |
Provider
From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.
Comments
Cre-TM mice (JAX 004682, B6.Cg-Tg(CAG-cre/Esr1)5Amc/J) were intercrossed with Lig3LoxP mice (Nature 471(2011) 240-244) to provide tamoxifen-inducible cre expression driven from the actin promoter. Lig3Cre-TM mouse embryonic fibroblast (MEF) cell cultures were prepared from E13.5 embryonic mesenchyme. Cells were allowed to proliferate in T-25 tissue culture flasks at 37 °C in a humidified CO2-regulated (5%) incubator and were immortalized with a retroviral construct 3315, which encodes SV40 large T antigen (J. Biol. Chem. 288 (2013) 26594-26605) and selected depending upon their ability to form colonies. One clone was selected for its superior growth characteristics and designated Cre4. This clone was transduced with a retrovirus 2641 (Mol. Biol. Rep. 37 (2010) 1987-1991, which encodes a Tet-On Advanced transactivator, EGFP and blasticidin resistance), thus producing a clone designated 4B6. 4B6 is a Tet-On derivative of the Cre4.
References
- Shokolenko IN, Fayzulin RZ, Katyal S, McKinnon PJ, Wilson GL, Alexeyev MF. Mitochondrial DNA ligase is dispensable for the viability of cultured cells but essential for mtDNA maintenance. J Biol Chem. 2013 Sep 13;288(37):26594-605.
If you publish research with this product, please let us know so we can cite your paper.
