Recombinant plasmid pBG68 produces Sindbis virus (SINV) replicons in cells that allows for fast and easy expression of your gene of interest.
Highlights:
The Sindbis virus genome was inserted into the pcDNA3.1 Zeo+ expression vector such that the transcription start site of the CMV promoter overlaps with the first viral nucleotide of the viral genome. A Hepatitis D virus (HDV) ribozyme was added to the 3' end of the viral genomic 35bp polyA sequence. The structural proteins from the 5' subgenomic promoter were replaced with the Blasticidin-S-deaminase (BSD) gene for replicon selection. A unique XbaI restriction site 3' subgenomic promoter to allow genes of interest to be ligated into the replicon. Transfection of this construct into mammalian cells produces a self-replicating Sindbis virus genome that expresses the BSD protein and any genes of interest cloned into the unique XbaI site, that can be packaged into single-round infectious virus-like particles by co-transfection with packaging plasmid pBG256 that can be used to insert the replicon into poorly or non-transfectable cells (in vitro or in vivo).
From the laboratory of Brian J. Geiss, PhD, Colorado State University.
Product Type: | Plasmid |
Gene/insert name: | Sindbis virus replicon |
Organism: | Sindbis Virus Strain TE3'2J (AR339) |
Antibiotic Resistance: | Amp |
Grow in E. coli at 37 C: | DH5-alpha or XL10-Gold E-coli |
Cloning Site 5': | MluI |
Cloning Site 3': | XhoI |
Insert Size: | 9,280 bp |
Vector Backbone and Size: | pcDNA3.1 Zeo+ |
High or low copy: | High |
Storage: | Room Temperature (Dried), -20C (liquid) |
Shipped: | Room Temperature |
If you publish research with this product, please let us know so we can cite your paper.