Hematopoietic-Myeloid Leukemia Cell Lines
The parental AML14 human myeloid leukemic cell line was established from a 68-year-old man who presented with FAB M2 acute myeloid leukemia.
AML14
- Morphologically, the cells appear quite undifferentiated
- Can be induced to eosinophilic differentiation with IL‐3, GM‐CSF, and IL‐5
AML14.3D10
- Fully differentiated eosinophilic myelocyte subline of the AML14
- Maintain advanced eosinophil phenotype and proliferates with a doubling time of 48 h in the absence of cytokine supplementation
- Cells will proliferate more rapidly in response to GM‐CSF or IL‐5
- AML14.3D10 longer expresses the gene for the α‐subunit of the IL‐3 receptor, compared to AML14
- Express mRNA and protein for all of the major eosinophil granule proteins (major basic protein [MBP], eosinophil derived neurotoxin [EDN], eosinophil cationic protein [ECP], eosinophil peroxidase [EPO]) and the cytosolic Charcot‐Leyden crystal protein (CLC)
AML14.eos
- Cytokine-induced fully differentiated eosinophilic myelocyte subline of AML14
- Proliferate and maintain a differentiated phenotype only if supplemented with cytokines (IL-3, Il-5, and GM-CSF)
- Express all of the major eosinophil granule proteins (major basic protein [MBP], eosinophil derived neurotoxin [EDN], eosinophil cationic protein [ECP], eosinophil peroxidase [EPO]) and the cytosolic Charcot‐Leyden crystal protein (CLC)
From the laboratories of Cassandra C. Paul, PhD and Michael A. Baumann, MD, Wright State University.
The parental AML14 human myeloid leukemic cell line was established from a 68-year-old man who presented with FAB M2 acute myeloid leukemia.
AML14
- Morphologically, the cells appear quite undifferentiated
- Can be induced to eosinophilic differentiation with IL‐3, GM‐CSF, and IL‐5
AML14.3D10
- Fully differentiated eosinophilic myelocyte subline of the AML14
- Maintain advanced eosinophil phenotype and proliferates with a doubling time of 48 h in the absence of cytokine supplementation
- Cells will proliferate more rapidly in response to GM‐CSF or IL‐5
- AML14.3D10 longer expresses the gene for the α‐subunit of the IL‐3 receptor, compared to AML14
- Express mRNA and protein for all of the major eosinophil granule proteins (major basic protein [MBP], eosinophil derived neurotoxin [EDN], eosinophil cationic protein [ECP], eosinophil peroxidase [EPO]) and the cytosolic Charcot‐Leyden crystal protein (CLC)
AML14.eos
- Cytokine-induced fully differentiated eosinophilic myelocyte subline of AML14
- Proliferate and maintain a differentiated phenotype only if supplemented with cytokines (IL-3, Il-5, and GM-CSF)
- Express all of the major eosinophil granule proteins (major basic protein [MBP], eosinophil derived neurotoxin [EDN], eosinophil cationic protein [ECP], eosinophil peroxidase [EPO]) and the cytosolic Charcot‐Leyden crystal protein (CLC)
From the laboratories of Cassandra C. Paul, PhD and Michael A. Baumann, MD, Wright State University.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
Specifications
| Product Type: | Cell Line |
| Cell Type: | Hematopoietic-Myeloid Leukemia |
| Morphology: |
AML14: Undifferentiated, resembles immature myeloid precursors. Exhibits eosinophilic differentiation in suspension in response to IL-3, IL-5, and GM-CSF. AML14.3D10 & AML14.eos: Proliferates as an eosinophilic myelocyte to metamyelocyte, containing eosinophil secondary granules. |
| Organism: | Homo sapiens, human |
| Source: | Peripheral blood |
| Biosafety Level: | BSL2 |
| Subculturing: | Grow in suspension (non-adherent). Maintain cell concentration between 3 x 105 to 1 x 106; do not allow concentration to exceed 1 x 106 cell/ml. Split cells every 4-5 days, 1 volume old culture:2 volumes fresh media. |
| Growth Conditions: | RPMI 1640, 8% FBS, 2mM L-glutamine, 1mM Sodium pyruvate, 50uM Beta-mercaptoethanol (see DOCUMENTATION section below) |
| Cryopreservation: | 90% FBS + 10% DMSO |
| Comments: |
AML14.3D10: When the density of 1x 106 cell/mL is exceeded, the cells appear to degranulate, which is fatal to the culture, presumably due to the toxic nature of eosinophil granule proteins. It is also difficult to resurrect the line from frozen aliquots for the same reason. Initial viability after thawing is usually about 20%‐30%. Recovery is facilitated by frequent replacement of culture medium during the first 72 h after thawing. AML14.eos: They exhibit adverse reactions to alkaline conditions and to serum with high iron content. If they start to revert to the parental strain they can be induced back to the eosinophilic mode with 100 pM GM-CSF, 250 pM IL-3 and 1 ng/mL IL-5. |
| Storage: | LN2 |
| Shipped: | Dry Ice |
Documentation
Provider
From the laboratories of Cassandra C. Paul, PhD and Michael A. Baumann, MD, Wright State University.
Comments
AML14.3D10: When the density of 1x 106 cell/mL is exceeded, the cells appear to degranulate, which is fatal to the culture, presumably due to the toxic nature of eosinophil granule proteins. It is also difficult to resurrect the line from frozen aliquots for the same reason. Initial viability after thawing is usually about 20%‐30%. Recovery is facilitated by frequent replacement of culture medium during the first 72 h after thawing.
AML14.eos: They exhibit adverse reactions to alkaline conditions and to serum with high iron content. If they start to revert to the parental strain they can be induced back to the eosinophilic mode with 100 pM GM-CSF, 250 pM IL-3 and 1 ng/mL IL-5.
AML14.eos: They exhibit adverse reactions to alkaline conditions and to serum with high iron content. If they start to revert to the parental strain they can be induced back to the eosinophilic mode with 100 pM GM-CSF, 250 pM IL-3 and 1 ng/mL IL-5.
References
- Paul C.C., Tolbert M., Mahrer S., Singh A., Grace M.J., Baumann M.A. Cooperative Effects of Interleukin-3 (IL-3), IL-5, and Granulocyte Macrophage Colony-Stimulating Factor: A New Myeloid Cell Line Inducible to Eosinophils. Blood, 81(5), 1993, pp 1193-1199
- Paul CC, Mahrer S, Tolbert M, Elbert BL, Wong I, Ackerman SJ, Baumann MA. Changing the differentiation program of hematopoietic cells: retinoic acid-induced shift of eosinophil-committed cells to neutrophils. Blood. 1995;86:3737-3744.
- Paul CC, Ackerman SJ, Mahrer S, Tolbert M, Dvorak AM, Baumann MA. Cytokine induction of granule protein synthesis in an eosinophil- inducible human myeloid cell line, AML14. J Leukoc Biol. 1994;56:74-79.
- Baumann M.A., Paul C.C.The AML14 and AML14.3D10 cell lines: a long-overdue model for the study of eosinophils and more. Stem Cells 16:16-24(1998)
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