EGFP-COSMO Plasmids
Plasmids for expression of chemically-induced dimerization (CID) system, caffeine-operated synthetic modules (COSMO), developed for chemogenetic control of many protein activities in live cells.
- Potent CID system; bivalent COSMO connected with a short/long linker also available
- Greatly enhances the affinity of acVHH to caffeine and its metabolites via high throughput screening
- Permits caffeine-inducible gating of calcium channels (enables control of calcium signaling and gene transcription)
- Remote control of transcription factor, and cancer cell killing
- EGFP-COSMO-PB Plasmid - GFP-tagged caffeine-operated synthetic module fused to polybasic domain (PB); enables caffeine-inducible cytosol-to-PM translocation
- EGFP-biCOSMO-S Plasmid - Bivalent COSMO connected with a short linker for caffeine-inducible dimerization
- EGFP-biCOSMO-L Plasmid - Bivalent COSMO connected with a long linker for caffeine-inducible allosteric control
Chemically Induced Dimerization (CID) is a biological mechanism in which two proteins bind only in the presence of a certain small molecule, enzyme or other dimerizing agent. Genetically engineered CID systems are useful in biological research fields for applications such as controlling protein localization, manipulating signaling pathways and inducing protein activation. COSMO anti-caffeine nanobody variants can be controlled by low-dose of caffeine and its metabolites.
From the laboratory of Yubin Zhou, MD, PhD, Texas A&M University.
Plasmids for expression of chemically-induced dimerization (CID) system, caffeine-operated synthetic modules (COSMO), developed for chemogenetic control of many protein activities in live cells.
- Potent CID system; bivalent COSMO connected with a short/long linker also available
- Greatly enhances the affinity of acVHH to caffeine and its metabolites via high throughput screening
- Permits caffeine-inducible gating of calcium channels (enables control of calcium signaling and gene transcription)
- Remote control of transcription factor, and cancer cell killing
- EGFP-COSMO-PB Plasmid - GFP-tagged caffeine-operated synthetic module fused to polybasic domain (PB); enables caffeine-inducible cytosol-to-PM translocation
- EGFP-biCOSMO-S Plasmid - Bivalent COSMO connected with a short linker for caffeine-inducible dimerization
- EGFP-biCOSMO-L Plasmid - Bivalent COSMO connected with a long linker for caffeine-inducible allosteric control
Chemically Induced Dimerization (CID) is a biological mechanism in which two proteins bind only in the presence of a certain small molecule, enzyme or other dimerizing agent. Genetically engineered CID systems are useful in biological research fields for applications such as controlling protein localization, manipulating signaling pathways and inducing protein activation. COSMO anti-caffeine nanobody variants can be controlled by low-dose of caffeine and its metabolites.
From the laboratory of Yubin Zhou, MD, PhD, Texas A&M University.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Plasmid |
| Gene/insert name: | anti-caffeine nanobody (COSMO), Bivalent COSMO connected with a short/long linker |
| Accession ID: | P42212 |
| Organism: | Synthetic |
| Antibiotic Resistance: | Kanamycin |
| Fusion Tag(s): | EGFP |
| Grow in E. coli at 37 C: | Yes |
| Selectable markers: | Neomycin |
| Cloning Site 5': | Agel |
| Cloning Site 3': | XhoI |
| Insert Size: | 354bp (COSMO-PB); 750bp (biCOSMO-S); 810bp (biCOSMO-L) |
| Vector Backbone and Size: | EGFP-C1, 4900 bp |
| High or low copy: | High |
| Storage: | -20C |
| Shipped: | Room temperature |
- Wang T, He L, Jing J, Lan TH, Hong T, Wang F, Huang Y, Ma G, Zhou Y. Caffeine-Operated Synthetic Modules for Chemogenetic Control of Protein Activities by Life Style. Adv Sci (Weinh). 2020 Dec 13;8(3):2002148. doi: 10.1002/advs.202002148. PMID: 33552855; PMCID: PMC7856909.
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