Yeast-Display Peptide Library
Yeast surface display library expressing randomized peptides with a broad use, such as in discovery of peptide ligands and binders to a wide variety of targets, screening for specific properties (eg. specific conformations) and more.
Highlights:
- Expresses randomized peptides of up to 8 amino acids in length as C-terminal fusions to a plasmid-encoded Aga2 tether protein
- Contains a diversity of approximately 6.1 × 109
- The expression construct features a hemagglutinin epitope tag (YPYDVPDYA) to monitor expression
- Useful for characterizing protein-peptide binding interactions, as well as to screen for other properties
- Compatible with Flow Cytometry
From the laboratory of Andrew C. Kruse, PhD, Harvard University.
Yeast surface display library expressing randomized peptides with a broad use, such as in discovery of peptide ligands and binders to a wide variety of targets, screening for specific properties (eg. specific conformations) and more.
Highlights:
- Expresses randomized peptides of up to 8 amino acids in length as C-terminal fusions to a plasmid-encoded Aga2 tether protein
- Contains a diversity of approximately 6.1 × 109
- The expression construct features a hemagglutinin epitope tag (YPYDVPDYA) to monitor expression
- Useful for characterizing protein-peptide binding interactions, as well as to screen for other properties
- Compatible with Flow Cytometry
From the laboratory of Andrew C. Kruse, PhD, Harvard University.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
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Customers CANNOT use this material for industrially sponsored academic research. Customers wishing to utilize this product for industrially sponsored academic research must first Contact Us before ordering.
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| Product Type: | Bacteria |
| Name: | Yeast-Display Peptide Library |
| Strain: | EBY100; (Type strain) |
| Organism: | Saccharomyces cerevisiae |
| Genotype: | AGA1::GAL1-AGA1::URA3 ura3-52 trp1 leu2-delta200 his3-delta200 pep4::HIS3 prbd1.6R can1 GAL |
| Mating Type: | MATa |
| Biosafety Level: | BSL-1 |
| Growth Conditions: | 30C; 25C under inducing conditions. Please see the SOP. |
| Format: | Frozen aliquot |
| Amount: | 1e10 cells per vial (5e9 cells/mL) |
| Comments: | Each vial contains 1e10 cells which offers ~80% library diversity. For selection campaigns that require sampling at a higher rate, 2 vials cover ~96.2%, while 3 vials cover ~99.3% of all clones in the library. Sampling from 6 vials covers >99.9% of the library. |
| Storage: | -80C |
| Shipped: | Dry Ice |
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