ASEC-1 Cell Lines
ASEC-1 is an immortalized lizard cell line derived from A. sagrei stage 6 embryo and can be used to study cellular processes (gene function) in reptile development and evolution.
Strong:
- Embryonic fibroblast cell line useful for studying reptile gene function and signaling pathways (such as genes with important roles in various organisms, Hedgehog signaling pathway)
- CRISPR/Cas9 knock-out ASEC-1 ift88-2/-4 and ASEC-1 ift88+1/-5 clones also available
From the laboratories of Douglas Menke, PhD and Jonathan Eggenschwiler, PhD, University of Georgia.
ASEC-1 is an immortalized lizard cell line derived from A. sagrei stage 6 embryo and can be used to study cellular processes (gene function) in reptile development and evolution.
Strong:
- Embryonic fibroblast cell line useful for studying reptile gene function and signaling pathways (such as genes with important roles in various organisms, Hedgehog signaling pathway)
- CRISPR/Cas9 knock-out ASEC-1 ift88-2/-4 and ASEC-1 ift88+1/-5 clones also available
From the laboratories of Douglas Menke, PhD and Jonathan Eggenschwiler, PhD, University of Georgia.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
Specifications
| Product Type: | Cell Line |
| Name: | ASEC-1 Cell Line, ASEC-1 ift88-2/-4 KO (clone 28), ASEC-1 ift88+1/-5 KO (clone 30) |
| Cell Type: | Fibroblast |
| Morphology: | Fibroblastic |
| Organism: | Anolis sagrei |
| Source: | Male, stage 6 embryo, torso (with no internal organs) |
| Biosafety Level: | BSL-1 |
| Growth Conditions: | 1X DMEM with 4.5 g/L glucose (without L- glutamine or sodium pyruvate; Corning 15-017-CM), 10% heat-inactivated fetal bovine serum (GeminiBio, Benchmark cat # 100-106), 1X glutamine (Corning 25-015-CI), 1X Penicillin/Streptomycin/Amphotericin B solution (Corning 30-004-CI) |
| Subculturing: | Doubling time: 20.7 hrs. Split Ratio: 1:3. Use 0.05% Trypsin-EDTA as the dissociation reagent. |
| Cryopreservation: | 10% DMSO, 15% FBS, 75% growth medium |
| Storage: | LN2 |
| Shipped: | Dry Ice |
Provider
From the laboratories of Douglas Menke, PhD and Jonathan Eggenschwiler, PhD, Department of Genetics, University of Georgia.
Comments
RNA-seq data and WGS data generated for ASEC-1 has been deposited in the Gene Expression Omnibus under accession GSE242114.
References
- Samudra SP, Park S, Esser EA, McDonald TP, Borges AM, Eggenschwiler J, Menke DB. A new cell culture resource for investigations of reptilian gene function. Development. 2024 Nov 15;151(22):dev204275. doi: 10.1242/dev.204275. Epub 2024 Nov 22. PMID: 39576177; PMCID: PMC11607698.
If you publish research with this product, please let us know so we can cite your paper.
