LacZ (CMV) Lentivirus
LacZ catalyzes the hydrolysis of the disaccharide lactose, into gluclose and galactose. The use of LacZ as a reporter gene, is due to its ability to cleave the bond between galactose and the indole, 5-bromo-4-chloro-3-hydroxyindole. The cleaved indole, is an easily detectable and insoluble blue compound.
This premade lentivirus includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction and expression of the target genes as well as antibiotics resistant genes or reporters. A woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) is believed to promote RNA processing events and nuclear export, boosting expression of your gene of interest in target cells by facilitating the production of mature mRNA from transcripts initiated by the virus's internal promoter (e.g. pCMV).
LacZ catalyzes the hydrolysis of the disaccharide lactose, into gluclose and galactose. The use of LacZ as a reporter gene, is due to its ability to cleave the bond between galactose and the indole, 5-bromo-4-chloro-3-hydroxyindole. The cleaved indole, is an easily detectable and insoluble blue compound.
This premade lentivirus includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction and expression of the target genes as well as antibiotics resistant genes or reporters. A woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) is believed to promote RNA processing events and nuclear export, boosting expression of your gene of interest in target cells by facilitating the production of mature mRNA from transcripts initiated by the virus's internal promoter (e.g. pCMV).
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Virus |
| Product MPN: | PLV-10001-50 |
| Biosafety Level: | BSL-2 |
| Vector Information: | Vector includes both 5? and 3? lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) |
| Promoter: | CMV |
| Selection Gene: | puromycin |
| Virus: | LV-CMV-LacZ |
| Titer: | 1 x 10^8CFU/ml |
| Storage: | Store at -80C. Multiple freeze/thaw cycles not recommended. |
| Shipped: | Dry ice |
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