Cy3 - maleimide
Cy3 is a bright, photostable, and pH insensitive fluorescent dye. Cy3 has been routinely used for labeling proteins, antibodies, and peptides. Cy3 conjugates give less background than TAMRA and most other fluorophores. Cy3 conjugates can be excited maximally at 550 nm, with peak emission at 570 nm. For fluorescence microscopy, Cy3 can be visualized with traditional TAMRA (tetramethylrhodamine) filter sets since the excitation and emission spectra are nearly identical to those of TAMRA.
Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or other appropriate reductant prior to the labeling.
Cy3 is a bright, photostable, and pH insensitive fluorescent dye. Cy3 has been routinely used for labeling proteins, antibodies, and peptides. Cy3 conjugates give less background than TAMRA and most other fluorophores. Cy3 conjugates can be excited maximally at 550 nm, with peak emission at 570 nm. For fluorescence microscopy, Cy3 can be visualized with traditional TAMRA (tetramethylrhodamine) filter sets since the excitation and emission spectra are nearly identical to those of TAMRA.
Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or other appropriate reductant prior to the labeling.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
Specifications
| Product Type: | Small Molecule |
| Chemical Formula: | C36H43N4O3BF4 |
| Molecular Weight: | 666.56 |
| Extinction Coefficient: | 150000 |
| Variant MPN: | 11080 |
| Format: | Red powder |
| Purity: | NMR 1H, HPLC-MS (95%) |
| Solubility: | well soluble in DMSO (0.50 M = 330 g/L), in DMF, dichloromethane, very poorly soluble in water (0.57 mM = 420 mg/L) |
| Spectral Information: | 555/570 nm |
| Storage: | Upon receipt store at -20C and desiccate |
| Shipped: | Ambient temperature |
References
- Schmidt, W.M.; Lehman, W.; Moore, J.R. Direct observation of tropomyosin binding to actin filaments. Cytoskeleton, 2015, 72(6), 292303. doi: 10.1002/cm.21225
- Guo, Y.; Yuan, H.; Claudio, N.M.; Kura, S.; Shakerdge, N.; Mempel, T.R.; Bacskai, B.J.; Josephson, L. PEG-Like Nanoprobes: Multimodal, Pharmacokinetically and Optically Tunable Nanomaterials. PLoS ONE, 2014, 9(4), e95406. doi: 10.1371/journal.pone.0095406
- Graen, T.M.D.; Hoefling, M.; Grubmüller, H. AMBER-DYES: Characterization of Charge Fluctuations and Force Field Parameterization of Fluorescent Dyes for Molecular Dynamics Simulations. Journal of Chemical Theory and Computation, 2014, 10(12), 5505-5512. doi: 10.1021/ct500869p
- Schulze, R.J.; Komar, J.; Botte, M.; Allen, W.J.; Whitehouse, S.; Gold, V.A.M.; Lycklama a Nijeholt, J.A.; Huard, K.; Berger, I.; Schaffitzel, C. et al. Membrane protein insertion and proton-motive-force-dependent secretion through the bacterial holo-translocon SecYEG-SecDF-YajC-YidC. Proceedings of the National Academy of Sciences of the U.S.A., 2014, 111(13), 4844-4849. doi: 10.1073/pnas.1315901111
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