Anti-Human Salivary Amylase [HABB] Antibody
This mouse IgG2a antibody was generated against purified human amylase and recognizes human salivary amylase and ovarian tumor amylase but not pancreatic amylase.
Alpha-Amylase is an enzyme (EC 3.2.1.1) that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. It is the major form of amylase found in humans and other mammals. Although found in many tissues, amylase is most prominent in pancreatic juice and saliva, each of which has its own isoform of human a-amylase. Salivary and Pancreatic amylases behave differently on isoelectric focusing, and can also be separated in testing by using specific monoclonal antibodies. Salivary amylase (also called ptyalin) breaks large, insoluble starch molecules into soluble starches (amylodextrin, erythrodextrin, and achrodextrin), producing successively smaller starches and ultimately maltose.
From a laboratory at University of Virginia.
This mouse IgG2a antibody was generated against purified human amylase and recognizes human salivary amylase and ovarian tumor amylase but not pancreatic amylase.
Alpha-Amylase is an enzyme (EC 3.2.1.1) that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. It is the major form of amylase found in humans and other mammals. Although found in many tissues, amylase is most prominent in pancreatic juice and saliva, each of which has its own isoform of human a-amylase. Salivary and Pancreatic amylases behave differently on isoelectric focusing, and can also be separated in testing by using specific monoclonal antibodies. Salivary amylase (also called ptyalin) breaks large, insoluble starch molecules into soluble starches (amylodextrin, erythrodextrin, and achrodextrin), producing successively smaller starches and ultimately maltose.
From a laboratory at University of Virginia.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Antibody |
| Antigen: | Human Salivary Amylase |
| Accession ID: | P04745 |
| Isotype: | IgG2a |
| Clonality: | Monoclonal |
| Clone Name: | HABB |
| Reactivity: | Reactive against human salivary amylase and ovarian tumor amylase but not pancreatic amylase |
| Immunogen: | Purified protein |
| Species Immunized: | Mouse |
| Purification Method: | Protein G |
| Buffer: | PBS, 0.05% (w/v) Sodium Azide |
| Tested Applications: | WB, IP |
| Concentration: | 1mg/mL |
| Storage: | 4C (working) -20C or -80C (stock) |
| Shipped: | Cold packs |
Specificity of Anti-Human Salivary Amylase (HABB) Monoclonal Antibody for salivary vs. pancreatic amylase
Electrophoresis of 50uL of a serum-based mixture of salivary (top) and pancreatic (bottom) amylases, with and without pretreatment with immobilized antibody to salivary amylase (Lane 1, purified untreated pancreatic and salivary amylase isoenzymes; Lane 2, 100 uL of antibody; Lanes 3, 4, 5, 6, and 7: samples contained 50, 20, 10, 5, 2 uL of antibody, respectively; Lane 8, 100 uL of saline. Adapted from: Mifflin TE, Benjamin DC and Bruns DE. Rapid quantitative, specific measurement of pancreatic amylase in serum with use of a monoclonal antibody. Clin Chem, 31(8):1283-1286 (1985).
- Mifflin TE, Benjamin DC and Bruns DE. Rapid quantitative, specific measurement of pancreatic amylase in serum with use of a monoclonal antibody. Clin Chem, 31(8):1283-1286 (1985).
- Bruns DE, Benjamin DC. Production and characterization of a monoclonal antibody to distinguish human pancreatic and salivary amylase. Clin Chem, 31(8):1286-1288 (1985).
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