This polyphosphate (High MW, p700) is heterogeneous in size, with approximate polymer lengths ranging from ~200-1,300 phosphate units; modal size is about 700 phosphate units.
Polyphosphate, a linear polymer of inorganic orthophosphate, which is widespread in living organisms and which plays diverse roles in biology, has recently been shown to be a potent modulator of the human blood clotting system. Its procoagulant activity is dependent on polymer length; this high MW polyphosphate preparation has very high specific activity in plasma clotting assays.
In addition to being a useful tool for plasma clotting assays, polyphosphate is can be used to study bacterial growth, virulence, and bacterial membrane function.
Also available:
From the laboratory of James H. Morrissey, PhD, University of Michigan.
Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
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Product Type: | Small Molecule |
Name: | Polyphosphate, Long Chain (p700) |
Chemical Formula: |
Nan+2PnO3n +1 or Lin+2PnO3n +1 n = the average number of phosphorus (P) atoms in the chain (Up to 10% of counterions may be Li+ rather than Na+) |
Molecular Weight: | Heterogeneous; Number average ~113 kDa; Range ~10 kDa to 208 kDa |
Format: | Lyophilized |
Purity: | <1% monophosphate |
Buffer: | 50mM MES, pH 6.0, 1mM EDTA (also see information in the product documentation) |
Solubility: | At least 1 M in water; <1 mM in buffers containing divalent metals (make intermediate dilutions in metal-free buffers) |
Concentration: | When reconstituted: 1 M (phosphate monomer) NOTE: To estimate the polymer concentration, divide the phosphate monomer concentration by the modal polymer length |
Size Distribution: | Mode of n: 1000-1300 (as measured by migration on PAGE) |
Storage: |
Dry material should be stored at room temperature (or lower) with dessication. Once reconstituted: - Stable at least 12 hours at room temperature - Stable at least 72 hours at 4C - Store long-term at -80C - Aliquot for repeated use, avoiding repeated freeze-thaw cycles - Do not store in the presence of divalent metal ions |
Shipped: | Ambient temperature |
Size Distribution
Polyphosphate has limited solubility in the presence of divalent metal ions, so make intermediate dilutions in water, or in buffers without divalent metals or polyphosphate-binding proteins.
Reconstitution:
1 - Centrifuge 100mg tube 1 min at 1000 x g to pellet material in bottom of tube.
2 - Reconstitute in 1.0 mL purified water to make a solution of 1 M phosphate (= 102 mg/mL polyP).
NOTE: May be reconstituted in larger volumes to make a more dilute solution as needed.
If you publish research with this product, please let us know so we can cite your paper.