Cy5 - Methyltetrazine
Cy5-Tetrazine is a fluorescent probe useful for chemoselective labeling and detection of TCO-tagged molecules.
Highlights:
- Biocompatible – click reaction occurs efficiently under mild buffer conditions; requires no accessory reagents such as a copper catalyst or reducing agents (e.g. DTT)
- Chemoselective – tetzines and trans-cyclooctene groups do not react or interfere with other functional groups found in biological samples but conjugate to one another with high efficiency
- Unprecedented kinetics – inverse-electron demand Diels-Alder chemistry is the fastest bioorthogonal ligation available, delivering conjugates within 30 min at 1 mg/mL concentrations
Tetrazine-activated fluorescent dyes are well suited for the detection of TCO-labeled biopolymers. The inverse-electron demand Diels-Alder cycloaddition reaction of TCO with tetrazines is a bioorthogonal reaction that possesses exceptional kinetics (k > 800 M-1s-1) and selectivity. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date.
Cy5-Tetrazine is a fluorescent probe useful for chemoselective labeling and detection of TCO-tagged molecules.
Highlights:
- Biocompatible – click reaction occurs efficiently under mild buffer conditions; requires no accessory reagents such as a copper catalyst or reducing agents (e.g. DTT)
- Chemoselective – tetzines and trans-cyclooctene groups do not react or interfere with other functional groups found in biological samples but conjugate to one another with high efficiency
- Unprecedented kinetics – inverse-electron demand Diels-Alder chemistry is the fastest bioorthogonal ligation available, delivering conjugates within 30 min at 1 mg/mL concentrations
Tetrazine-activated fluorescent dyes are well suited for the detection of TCO-labeled biopolymers. The inverse-electron demand Diels-Alder cycloaddition reaction of TCO with tetrazines is a bioorthogonal reaction that possesses exceptional kinetics (k > 800 M-1s-1) and selectivity. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
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Specifications
| Product Type: | Small Molecule |
| Name: | Cy5-Tetrazine |
| Chemical Formula: | C44H51N7O10S3(protonated) |
| Molecular Weight: | 934.10 (protonated) |
| Variant MPN: | 1019 |
| Purity: | >95%, HPLC |
| Solubility: | DMSO, DMF, Water |
| Storage: | -20C.Desiccate |
| Shipped: | Ambient |
Documentation
If you publish research with this product, please let us know so we can cite your paper.
References
- Koshy ST, Desai RM, Joly P, Li J, Bagrodia RK, Lewin SA, Joshi NS, Mooney DJ. Click-Crosslinked Injectable Gelatin Hydrogels. Adv Healthc Mater. 2016 Mar 9;5(5):541-7. doi: 10.1002/adhm.201500757. PubMed PMID: 26806652; PubMed Central View Article
If you publish research with this product, please let us know so we can cite your paper.
