miR-302 (Inducible Tet-On) Lentivirus
This premade lentivirus includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction and expression of the target genes as well as antibiotics resistant genes or reporters. A woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) is believed to promote RNA processing events and nuclear export, boosting expression of your gene of interest in target cells by facilitating the production of mature mRNA from transcripts initiated by the virus's internal promoter (e.g., pCMV).
This premade lentivirus includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction and expression of the target genes as well as antibiotics resistant genes or reporters. A woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) is believed to promote RNA processing events and nuclear export, boosting expression of your gene of interest in target cells by facilitating the production of mature mRNA from transcripts initiated by the virus's internal promoter (e.g., pCMV).
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Virus |
| Product MPN: | PLV-10024-50 |
| Biosafety Level: | BSL-2 |
| Vector Information: | Vector includes both 5? and 3? lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) |
| Target Gene / Reporter(s): | miR-302 |
| Promoter: | TRE |
| Selection Gene: | Puromycin |
| Virus: | miR-302 (Inducible Tet-On) Lentivirus |
| Titer: | 1 x 10^8CFU/mL |
| Storage: | Store at -80C. Multiple freeze/thaw cycles not recommended. |
| Shipped: | Dry ice |
NOTE: This Inducible Tet-On lentivirus does not contain the reverse tetracycline-controlled transactivator (rtTA) protein, which is required for the Tet-On system. Unless the cells used stably produce rtTA, it is necessary to cotransduce this lentivirus with a rtTA lentivirus (rtTA (CMV, neomycin) Lentivirus or rtTA (CMV, puromycin) Lentivirus.
1.Blair JD, Bateup HS. New frontiers in modeling tuberous sclerosis with human stem cell-derived neurons and brain organoids. Dev Dyn. 2020 Jan;249(1):46-55. View article
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