An azide-reactive desthiobiotin reagent that allows easy eluion from streptavidin.
Highlights:
Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.
Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
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Product Type: | Small Molecule |
Name: | DBCO-PEG4-Desthiobiotin |
Chemical Formula: | C39H51N5O8 |
Molecular Weight: | 719.87 |
Variant MPN: | 1108 |
Format: | Colorless to slightly yellow oil |
Solubility: | DMSO, DMF, MeOH, DCM, THF |
Storage: | -20C. Desiccate |
Shipped: | Frozen overnight - Ambient |
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