TAMRA-Azide-Desthiobiotin
An analog of TAMRA-Azide-Biotin trifunctional reagent that allows for easy elution from streptavidin.
Highlights:
- Trifunctional – allows for both visualization and affinity purification of alkyne-tagged proteins
- Chemoselective – azide and alkyne groups do not react or interfere with other functional groups found in biological samples, but conjugate to one another with high efficiency
- Desthiobiotin – allows easy elution from streptavidin, an ideal feature for affinity purification applications
- Solubility – reagent easily dissolves in water-miscible solvents for subsequent dilution into aqueous reaction mixtures
Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.
An analog of TAMRA-Azide-Biotin trifunctional reagent that allows for easy elution from streptavidin.
Highlights:
- Trifunctional – allows for both visualization and affinity purification of alkyne-tagged proteins
- Chemoselective – azide and alkyne groups do not react or interfere with other functional groups found in biological samples, but conjugate to one another with high efficiency
- Desthiobiotin – allows easy elution from streptavidin, an ideal feature for affinity purification applications
- Solubility – reagent easily dissolves in water-miscible solvents for subsequent dilution into aqueous reaction mixtures
Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Small Molecule |
| Name: | TAMRA-Azide-Desthiobiotin |
| Chemical Formula: | C57H82N11O14 |
| Molecular Weight: | 1145.33 |
| Extinction Coefficient: | 92000 M-1cm-1 |
| Variant MPN: | 1110 |
| Format: | Red amorphous solid |
| Solubility: | DMSO, DMF, water, MeOH |
| Spectral Information: | Abs/Em = 546/565 nm |
| Storage: | -20C |
| Shipped: | Ambient |
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