DNA-Binding Fluorescent Stain (Miami Yellow)
This fluorescent nuclear dye has been shown to be brighter than traditional nuclear stains (e.g., DAPI, Hoechst) in Human Embryonic Kidney (HEK) and breast cancer cells (MCF7). It is ideal for in vivo cell or tissue studies where prolonged imaging experiments are necessary.
Highlights:
- Low cytotoxicity, membrane permeable, and live-cell compatible fluorescent probe
- Increased sensitivity - Allows for lower dye loadings and/or illuminations intensities
- π (Pi)-electronic system – Enables excitation at visible wavelengths
- Rotatable arms – Permit for detection at common fluorescence microscope wavelengths
- Cationic moieties – Allow for efficient intercalation into DNA
From the laboratory of James N. Wilson, PhD, University of Miami.
Part of The Investigator's Annexe program.
This fluorescent nuclear dye has been shown to be brighter than traditional nuclear stains (e.g., DAPI, Hoechst) in Human Embryonic Kidney (HEK) and breast cancer cells (MCF7). It is ideal for in vivo cell or tissue studies where prolonged imaging experiments are necessary.
Highlights:
- Low cytotoxicity, membrane permeable, and live-cell compatible fluorescent probe
- Increased sensitivity - Allows for lower dye loadings and/or illuminations intensities
- π (Pi)-electronic system – Enables excitation at visible wavelengths
- Rotatable arms – Permit for detection at common fluorescence microscope wavelengths
- Cationic moieties – Allow for efficient intercalation into DNA
From the laboratory of James N. Wilson, PhD, University of Miami.
Part of The Investigator's Annexe program.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
Specifications
| Product Type: | Small Molecule |
| Name: | Miami Yellow ("2" in reference) |
| Chemical Formula: | C25H31BF2N4O2 |
| Molecular Weight: | 468.4 |
| Format: | Yellow liquid |
| Purity: | > 95% by 1HNMR |
| Solubility: | Soluble in DMSO up to at least 1.5mM, soluble in aqueous solutions up to 5uM |
| Spectral Information: | λ max, ex (DNA bound) = 502nm, λ max, em = 558nm |
| Platform: | Confocal microscope, Fluorometer |
| Compatible Cells: | HEK293, MCF7, BT474 (others not tested) |
| Detection Method: | Fluorescence |
| Comments: | Suggested working concentration 1-2uM |
| Storage: | 4C (protect from light) |
| Shipped: | Ambient temperature |
Data
Imaging Parameters
(A) Excitation and emission spectra of Miami Yellow. (B) Representative image of cells (MCF7) treated with Miami Yellow.
Provider
From the laboratory of James N. Wilson, PhD, University of Miami.
References
- Pitter DR, Wigenius J, Brown AS, Baker JD, Westerlund F, Wilson JN. Turn-on, fluorescent nuclear stains with live cell compatibility. Org Lett. 2013 Mar 15;15(6):1330-3.
- Wilson JN, Wigenius J, Pitter DR, Qiu Y, Abrahamsson M, Westerlund F. Base pair sensitivity and enhanced ON/OFF ratios of DNA-binding: donor-acceptor-donor fluorophores. J Phys Chem B. 2013 Oct 10;117(40):12000-6.
- Quyen TL, Ngo TA, Bang DD, Madsen M, Wolff A. Classification of Multiple DNA Dyes Based on Inhibition Effects on Real-Time Loop-Mediated Isothermal Amplification (LAMP): Prospect for Point of Care Setting. Front Microbiol. 2019 Oct 15;10:2234. View article
