Human S100A8/A9 Heterodimer (Calprotectin)

Recombinant human S100A8/A9 heterodimer complex (Calprotectin) protein expressed in and purified from E. coli.

Highlights:

  • Highly pure (>95%)
  • Fully functional - Quality control growth assays on each batch confirm protein activity
  • Endotoxin free version available - Ideal for cell-based studies

Calprotectin (CP), also known as MRP-8/MRP-14 or S100A8/A9 heterocomplex, is a critical factor in the innate immune response to a wide range of microbial pathogens. CP functions in this role via a mechanism termed nutritional immunity, by sequestering essential transition metals required for growth and vitality. Recent literature has identified instances where over-accumulation of calprotectin can play a key role in inflammatory diseases, multiple forms of arthritis, tumor metastasis and sepsis. In the inflammatory response it has been shown that CP binding to extracellular receptors like pattern recognition proteins RAGE (Receptor for Advanced Glycation Endproducts) and Toll-like receptors (TLR) induce a positive feedback loop that promotes infection.

From the laboratory of Walter J. Chazin, PhD, Vanderbilt University.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EVU201
Human S100A8/A9 Heterodimer (Calprotectin), 50ug
50ug, 12.2mg/mL In stock
Regular Price:$355.00
On Sale:
EVU204
Human S100A8/A9 Heterodimer (Calprotectin), 500ug (Endotoxin-Free)
500ug, 8.3mg/mL (Endotoxin-Free) In stock
Regular Price:$2,960.00
On Sale:
EVU203
Human S100A8/A9 Heterodimer (Calprotectin), 500ug
500ug, 12.2mg/mL In stock
Regular Price:$2,370.00
On Sale:
EVU202
Human S100A8/A9 Heterodimer (Calprotectin), 50ug (Endotoxin-Free)
50ug, 8.3mg/mL (Endotoxin-Free) In stock
Regular Price:$445.00
On Sale:
Specifications

Product Type: Protein
Name: Calprotectin, MRP-8/MRP-14, S100A8/A9
Accession ID: P05109, P06702, HS07074, HS08809
Source: Recombinant (E. coli), bacterial overexpression
Molecular Weight: 23,945 Da
Amino Acid Sequence: MLTELEKALNSIIDVYHKYSLIKGNFHAVYRDDLKKLLETECPQYIRKKGADVWFKELDINTDGAVNFQEFLILVIKMGVAAHKKSHEESHKE/TCKMSQLERNIETIINTFHQYSVKLGHPDTLNQGEFKELVRKDLQNFLKKENKNEKVIEHIMEDLDTNADKQLSFEEFIMLMARLTWASHEKMHEGDEGPGHHHKPGLGEGTP
Purity: >95%, endotoxin-free
Buffer: 20 mM Tris pH 8.0, 100 mM NaCl, 10 mM ?-mercaptoethanol
Concentration: 8.3mg/mL (EVU202, EVU204); 12.2mg/mL (EVU201, EVU203)
Comments: Quality control growth assays are performed on each batch of protein, ensuring that the protein is active
Storage: -80C
Shipped: Dry ice

Provider
From the laboratory of Walter J. Chazin, PhD, Vanderbilt University.
References
  1. Kehl-Fie TE, Chitayat S, Hood MI, Damo S, Restrepo N, Garcia C, Munro KA, Chazin WJ, Skaar EP. Nutritional metal sequestration by calprotectin enhances neutrophil killing of Staphylococcus aureus through inhibition of superoxide defense. Cell Host and Microbe 10, 158-164 (2011).
  2. Damo SM, Kehl-Fie TE, Sugitani N, Holt ME, Rathi S, Murphy WJ, Zhang Y, Betz C, Hench L, Fritz G, Skaar EP, Chazin WJ. Molecular basis for manganese sequestration by calprotectin and roles in the innate immune response to invading bacterial pathogens". Proc. Natl. Acad. Sci., USA 110, 3841-3846 (2013).

If you publish research with this product, please let us know so we can cite your paper.

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