Brainbow Antibodies
These polyclonal antibodies were designed for use with Brainbow applications, and are capable of distinguishing between individual neurons in the brain.
Highlights:
- Green fluorescent protein (GFP), mTFP, mCherry, and red (orange) fluorescent protein (TagRFP) antibodies avaliable
- Tested with mouse, drosophila and zebrafish tissues
- Suitable for fluorescent imaging applications
The brainbow process works by randomly expressing different ratios of red, green, and blue derivatives of green fluorescent protein in individual neurons, it is possible to flag each neuron with a distinctive color. This process has been a major contribution to the field of connectomics, or the study of neural connections in the brain.
From the laboratory of Dawen Cai, PhD, University of Michigan.
Part of The Investigator's Annexe program.
These polyclonal antibodies were designed for use with Brainbow applications, and are capable of distinguishing between individual neurons in the brain.
Highlights:
- Green fluorescent protein (GFP), mTFP, mCherry, and red (orange) fluorescent protein (TagRFP) antibodies avaliable
- Tested with mouse, drosophila and zebrafish tissues
- Suitable for fluorescent imaging applications
The brainbow process works by randomly expressing different ratios of red, green, and blue derivatives of green fluorescent protein in individual neurons, it is possible to flag each neuron with a distinctive color. This process has been a major contribution to the field of connectomics, or the study of neural connections in the brain.
From the laboratory of Dawen Cai, PhD, University of Michigan.
Part of The Investigator's Annexe program.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
Specifications
| Product Type: | Antibody |
| Antigen: | mCherry |
| Accession ID: | X5DSL3 |
| Species Immunized: | Chicken |
Provider
From the laboratory of Dawen Cai, PhD, University of Michigan.
Comments
- anti-GFP: Antibody recognizes fluorescent proteins originated from jellyfish (Aequorea victoria), tested on mAmetrine, EBFP2, ECFP, Cerulean, EGFP, sfGFP, EYFP, mCitrine and Venus. No detectable cross-reactivity when used together with fluorescent proteins listed in the other forms.
- anti-mCherry: Antibody recognizes fluorescent proteins originated from coral (Discosoma sp.), tested on mOrange2, tdTomato, mCherry, DsRed. No detectable cross-reactivity when used together with fluorescent proteins listed in the other forms.
- anti-mTFP: Antibody recognizes fluorescent proteins originated from coral (Clavularia sp.), tested on mTFP and mWasabi. No detectable cross-reactivity when used together with fluorescent proteins listed in the other forms.
- anti-TagRFP: Antibody recognizes fluorescent proteins originated from sea anemone (Entacmaea quadricolor), tested on TagBFP, TagRFP, TagRFPt, mKate2, tdKatushka2. No detectable cross-reactivity when used together with fluorescent proteins listed in the other forms.
References
- Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR. Improved tools for the Brainbow toolbox. Nat Methods. 2013 May 5;10(6):540-7.
- Tillberg PW, Chen F, Piatkevich KD, Zhao Y, Yu CJ, English BP, Gao L, Martorell A, Suk HJ, Yoshida F, DeGennaro EM, Roossien DH, Gong G, Seneviratne U, Tannenbaum SR, Desimone R, Cai D, Boyden ES. Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies. Nat Biotechnol 2016;34(9):987-992
- Chang JB, Chen F, Yoon YG, Jung EE, Babcock H, Kang JS, Asano S, Suk HJ, Pak N, Tillberg PW, Wassie AT, Cai D, Boyden ES. Iterative expansion microscopy. Nat Methods. 2017 Apr 17. doi: 10.1038/nmeth.4261. [Epub ahead of print] PubMed PMID: 28417997. View Article
- Manent J, Banerjee S, de Matos Simoes R, Zoranovic T, Mitsiades C, Penninger JM, Simpson KJ, Humbert PO, Richardson HE. Autophagy suppresses Ras-driven epithelial tumourigenesis by limiting the accumulation of reactive oxygen species. Oncogene. 2017 Jun 5. View Article
- Chen CC, Lu J, Yang R, Ding JB, Zuo Y. Selective activation of parvalbumin interneurons prevents stress-induced synapse loss and perceptual defects. Mol Psychiatry. 2017 Aug 1. doi: 10.1038/mp.2017.159. [Epub ahead of print] View Article
- Zanca C, Villa GR, Benitez JA, Thorne AH, Koga T, D'Antonio M, Ikegami S, Ma J, Boyer AD, Banisadr A, Jameson NM, Parisian AD, Eliseeva OV, Barnabe GF, Liu F, Wu S, Yang H, Wykosky J, Frazer KA, Verkhusha VV, Isaguliants MG, Weiss WA, Gahman TC, Shiau AK, Chen CC, Mischel PS, Cavenee WK, Furnari FB. Glioblastoma cellular cross-talk converges on NF-κB to attenuate EGFR inhibitor sensitivity. Genes Dev. 2017 Jul 19. doi: 10.1101/gad.300079.117. [Epub ahead of print] View Article
If you publish research with this product, please let us know so we can cite your paper.
