L-Homopropargylglycine
L-Homopropargylglycine (HPG) is an amino acid analog of methionine that contains a very small modification, specifically an alkyne moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis.
Detection utilizes the chemoselective ligation or “click " reaction between an azide and an alkyne or cyclooctyne. The alkyne-modified protein is detected with either fluorescent azide or biotin azide. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS⁄MS and MALDI MS analysis.
L-Homopropargylglycine (HPG) is an amino acid analog of methionine that contains a very small modification, specifically an alkyne moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis.
Detection utilizes the chemoselective ligation or “click " reaction between an azide and an alkyne or cyclooctyne. The alkyne-modified protein is detected with either fluorescent azide or biotin azide. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS⁄MS and MALDI MS analysis.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
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Specifications
| Product Type: | Small Molecule |
| Chemical Formula: | C6H10ClNO2 |
| CAS number: | 98891-36-2 |
| Molecular Weight: | 163.60 (HCl salt) |
| Variant MPN: | 1067 |
| Format: | White crystalline |
| Purity: | >95% (HPLC) |
| Solubility: | Water, DMSO, DMF |
| Storage: | -20C |
| Shipped: | Ambient |
References
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