OSKM (Inducible Tet-On) Lentivirus

LV-EF1A-miR211

This product packages all four genes (OSKM) in one lentiviral vector. All four genes are expressed under the control of Tet promoter.

Induced pluripotent stem (iPS) cells can be generated from differentiated somatic cells by over-expressing a set of specific transcription factors called reprogramming factors. Typically four reprogramming factors are chosen, Oct4, Sox2, Klf4 and c-Myc (OSKM), which are introduced into the target cells through genetic transduction using retroviral or lentiviral vectors.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
FCT212
OSKM (Inducible Tet-On) Lentivirus, 2x25ul
2x25uL (1x10^8 CFU/mL) In stock
Regular Price:$665.00
On Sale:
FCT213
OSKM (Inducible Tet-On) Lentivirus, 8x25ul
8x25uL (1x10^8 CFU/mL) In stock
Regular Price:$1,525.00
On Sale:
Specifications

Product Type: Virus
Product MPN: PLV-10091-50
Biosafety Level: BSL-2
Vector Information: Vector includes both 5? and 3? lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE)
Target Gene / Reporter(s): OSKM
Promoter: TRE
Selection Gene: N/A
Titer: 1 x 10^8TU/mL
Storage: Store at -80C. Multiple freeze/thaw cycles not recommended.
Shipped: Dry ice

Documentation
Comments

NOTE: This Inducible Tet-On lentivirus does not contain the reverse tetracycline-controlled transactivator (rtTA) protein, which is required for the Tet-On system. Unless the cells used stably produce rtTA, it is necessary to cotransduce this lentivirus with a rtTA lentivirus (rtTA (CMV, neomycin) Lentivirus or rtTA (CMV, puromycin) Lentivirus.

References

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