Dimedone-d6
Dimedone-d6 is a deuterated version of dimedone, a chemoselective probe for detecting sulfenylated proteins in intact cells.
Highlights:
- Consists of 5,5-dimethyl-1,3-cyclohexanedione where two methyl groups are fully deuterated
- Used along with dimedone (or iododimedone) for the quantification of sulfenic acid
- Permits S-sulfenylation site occupancy to be monitored at individual cysteines within a single protein and is compatible with peptide-based proteomic strategies
Mild oxidation can convert the sulfhydryl group of cysteine residues (Cys-SH) on proteins to cysteine-sulfenic acid derivatives (Cys-SOH). Protein sulfenylation, then, is a post-translational modification that is relevant to redox signaling.
From the laboratory of Kate Carroll, PhD, The Scripps Research Institute.
Dimedone-d6 is a deuterated version of dimedone, a chemoselective probe for detecting sulfenylated proteins in intact cells.
Highlights:
- Consists of 5,5-dimethyl-1,3-cyclohexanedione where two methyl groups are fully deuterated
- Used along with dimedone (or iododimedone) for the quantification of sulfenic acid
- Permits S-sulfenylation site occupancy to be monitored at individual cysteines within a single protein and is compatible with peptide-based proteomic strategies
From the laboratory of Kate Carroll, PhD, The Scripps Research Institute.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
Specifications
| Product Type: | Small Molecule |
| Name: | 5,5-bis(methyl-d3)cyclohexane-1,3-dione (Dimedone ?d6) |
| Chemical Formula: | C8H6D6O2 |
| Molecular Weight: | 146.22 |
| Format: | Pale yellow solid |
| Purity: | >98%, HPLC |
| Solubility: | Stock solution in DMSO (up to 500 mM), then dilute to appropriate concentration in aqueous buffer with final DMSO concentration < 5% |
| Spectral Information: | 1H NMR (DMSO-d6, 400 MHz), δ 10.95 (s, 1H), 5.19 (s, 1H), 2.11(s, 4H). 13C NMR (DMSO-d6, 100 MHz): δ102.51, 102.50, 46.38, 31.80, 27.19. EI- LRMS calcd. For C8H6D6O2 [M]+ 146.1214, found 146.1215 |
| Storage: | Room temperature for short term, -20C for long term storage |
| Shipped: | Dry ice |
Provider
From the laboratory of Kate Carroll, PhD, The Scripps Research Institute.
References
- Seo YH, Carroll KS. Quantification of protein sulfenic acid modifications using isotope-coded dimedone and iododimedone. Angew Chem Int Ed Engl. 2011 Feb 7;50(6):1342-5. doi: 10.1002/anie.201007175.
- Gupta, V.; Carroll, K. S. Sulfenic Acid Chemistry, Detection and Cellular Lifetime. Biochimica et Biophysica Acta - General Subjects 2014, 1840, 847-875
- Garcia FJ, Carroll KS. An immunochemical approach to detect oxidized protein tyrosine phosphatases using a selective C-nucleophile tag. Mol Biosyst. 2016 Jan 13. PubMed PMID: 26757830.
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