PGC-1α/ERRα Pathway HEK-293T Cell Lines
HEK293T/17 Cells expressing endogenous estrogen-related receptor α (ERRα) transduced with peroxisome proliferator-activated receptor c (PPARc) coactivator 1α (PGC-1α). Also available, ERRα transduced and ERRα/PGC-1α transduced versions.
Highlights
- 293T-PGC Cell Line - HEK293T/17 cells that express endogenous ERRα were transduced with the lentiviral PGC-1α expression construct to create this stable clonal cell line
- ERR Reporter Cell Line - HEK293T/17 cells were transduced with the lentiviral ERRα multiple hormone response element (MHRE) reporter expression construct to create this stable clonal cell line
- PGC/ERR Reporter Cell Line - HEK293T/17 cells that express endogenous ERRα were transduced with lentiviral PGC-1α expression construct and ERRα multiple hormone response element (MHRE) reporter to create this stable cell line
The estrogen-related receptor a (ERRα) and the peroxisome proliferator-activated receptor c (PPARc) coactivator 1a (PGC-1α) play critical roles in the control of several physiological functions, including the regulation of genes involved in energy homeostasis.
From the laboratory of Christina T. Teng, PhD, National Institute of Environmental Health Sciences/NIH.
HEK293T/17 Cells expressing endogenous estrogen-related receptor α (ERRα) transduced with peroxisome proliferator-activated receptor c (PPARc) coactivator 1α (PGC-1α). Also available, ERRα transduced and ERRα/PGC-1α transduced versions.
Highlights
- 293T-PGC Cell Line - HEK293T/17 cells that express endogenous ERRα were transduced with the lentiviral PGC-1α expression construct to create this stable clonal cell line
- ERR Reporter Cell Line - HEK293T/17 cells were transduced with the lentiviral ERRα multiple hormone response element (MHRE) reporter expression construct to create this stable clonal cell line
- PGC/ERR Reporter Cell Line - HEK293T/17 cells that express endogenous ERRα were transduced with lentiviral PGC-1α expression construct and ERRα multiple hormone response element (MHRE) reporter to create this stable cell line
The estrogen-related receptor a (ERRα) and the peroxisome proliferator-activated receptor c (PPARc) coactivator 1a (PGC-1α) play critical roles in the control of several physiological functions, including the regulation of genes involved in energy homeostasis.
From the laboratory of Christina T. Teng, PhD, National Institute of Environmental Health Sciences/NIH.
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| Product Type: | Cell Line |
| Cell Type: | HEK 293T/17 (ATCC #CRL-11268) |
| Accession ID: | Q9UBK2 |
| Organism: | Human |
| Morphology: | Epithelium |
| Source: | Embryonic kidney |
| Biosafety Level: | BSL-2 |
| Subculturing: | Yes |
| Growth Conditions: |
293T-PGC Cell Line: High-glucose Dulbecco’s Modified Essential Medium (DMEM), supplemented with 1 mM N-pyruvate, 4 mM glutamine and 10% fetal bovine serum (FBS), 100 U Penicillin, 0.1 mg/ml Streptomycin, and 1 ug/mL puromycin at 37 C under 5% CO2 ERR Reporter Cell Line: High-glucose Dulbecco’s Modified Essential Medium (DMEM), supplemented with 4 mM glutamine, 1 mM sodium pyruvate and 10% fetal bovine serum (FBS), 100 U penicillin, and 100 ug/mL streptomycin at 37 C under 5% CO2 PGC/ERR Reporter Cell Line: High-glucose Dulbecco’s Modified Essential Medium (DMEM), supplemented with 4 mM glutamine, 1 mM sodium pyruvate and 10% fetal bovine serum (FBS), 100 U penicillin, 100 ug/mL streptomycin, and 1 ug/mL puromycin at 37 C under 5% CO2 |
| Cryopreservation: | Standard |
| Storage: | Liquid nitrogen |
| Shipped: | Dry Ice |
- Teng CT, Hsieh JH, Zhao J, Huang R, Xia M, Martin N, Gao X, Dixon D, Auerbach SS, Witt KL, Merrick BA. Development of Novel Cell Lines for High-Throughput Screening to Detect Estrogen-Related Receptor Alpha Modulators. SLAS Discov. 2017 Jan 1:2472555216689772. doi: 10.1177/2472555216689772. [Epub ahead of print] PubMed PMID: 28346099.
- Liu D, Zhang Z, Gladwell W, Teng CT. Estrogen stimulates estrogen-related receptor alpha gene expression through conserved hormone response elements. Endocrinology. 2003 Nov;144(11):4894-904. Epub 2003 Jul 24. PubMed PMID: 12960079.
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