Pyrene phosphoramidite dU
This pyrene phosphoramidite contains hydrocarbon moiety rigidly attached to deoxyuridine. This reagent allows to attach pyrene fragment to 5', internal, or 3' position (using universal support).
Pyrene is polycyclic aromatic hydrocarbon which is well-known for its ability to intercalate into DNA. Pyrene features intense blue fluorescence. Its parameters depend strongly on fluorophore microenvironment. Therefore, fluorescence spectra of pyrene are used for the extraction of structural information about site surrounding pyrene. Two pyrenes in close proximity usually form excimers easily detectable by excimer fluorescence. Pyrene can also be a FRET donor to other fluorophores such as perylene.
With this phosphoramidite, pyrene can be introduced into DNA or RNA by means of automated synthesis. This amidite requires no special handling, coupling, or deprotection conditions. Recommended diluent for it is acetonitrile.
This pyrene phosphoramidite contains hydrocarbon moiety rigidly attached to deoxyuridine. This reagent allows to attach pyrene fragment to 5', internal, or 3' position (using universal support).
Pyrene is polycyclic aromatic hydrocarbon which is well-known for its ability to intercalate into DNA. Pyrene features intense blue fluorescence. Its parameters depend strongly on fluorophore microenvironment. Therefore, fluorescence spectra of pyrene are used for the extraction of structural information about site surrounding pyrene. Two pyrenes in close proximity usually form excimers easily detectable by excimer fluorescence. Pyrene can also be a FRET donor to other fluorophores such as perylene. With this phosphoramidite, pyrene can be introduced into DNA or RNA by means of automated synthesis. This amidite requires no special handling, coupling, or deprotection conditions. Recommended diluent for it is acetonitrile.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Small Molecule |
| Chemical Formula: | C57H55N4O8P |
| Molecular Weight: | 955.04 |
| Variant MPN: | 11460 |
| Format: | yellowish foam |
| Purity: | NMR 1H (95%), 31P, HPLC-MS |
| Solubility: | good in dichloromethane and acetonitrile |
| Spectral Information: |
Excitation maximum, nm: 260, 282, 365, 392 Emission maximum, nm: 460 |
| Quantum Yield: | ~0.1 |
| Storage: | 24 months after receival at -20°C in the dark. |
| Shipped: | room temperature |
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