KRAS4b Baculovirus Expression Vectors
These plasmids are pFastBac style baculovirus expression vectors in which human KRAS is expressed from the baculovirus polyhedrin promoter with a maltose binding protein solubility tag.
- KRAS gene is full-length including C-terminal CVIM sequence targeting the protein for proper farnesylation and membrane localization when produced in insect cells with coexpressed farnesyltransferase
- Amino terminal His6 tag allows for easy downstream purification
- TEV protease cleavage site downstream of the MBP fusion permits tag removal (leaving single additional glycine residue on the amino terminal end of the final KRAS protein)
- Plasmids differ only in the mutation in the human KRAS gene
- Most useful when coupled with: Baculovirus AcMNPV Expressing E. coli Cell Line (DE35)
KRAS gene makes a protein called K-Ras that is involved primarily in regulating cell division. As part of a signaling pathway known as the RAS/MAPK pathway, the protein relays signals from outside the cell to the cell's nucleus. These signals instruct the cell to grow and divide or to mature and take on specialized functions (differentiate). The KRAS gene is also an oncogene, when mutated normal cells can be become cancerous.
From the laboratory of Dominic Esposito, PhD, National Cancer Institute/NIH.
These plasmids are pFastBac style baculovirus expression vectors in which human KRAS is expressed from the baculovirus polyhedrin promoter with a maltose binding protein solubility tag.
- KRAS gene is full-length including C-terminal CVIM sequence targeting the protein for proper farnesylation and membrane localization when produced in insect cells with coexpressed farnesyltransferase
- Amino terminal His6 tag allows for easy downstream purification
- TEV protease cleavage site downstream of the MBP fusion permits tag removal (leaving single additional glycine residue on the amino terminal end of the final KRAS protein)
- Plasmids differ only in the mutation in the human KRAS gene
- Most useful when coupled with: Baculovirus AcMNPV Expressing E. coli Cell Line (DE35)
KRAS gene makes a protein called K-Ras that is involved primarily in regulating cell division. As part of a signaling pathway known as the RAS/MAPK pathway, the protein relays signals from outside the cell to the cell's nucleus. These signals instruct the cell to grow and divide or to mature and take on specialized functions (differentiate). The KRAS gene is also an oncogene, when mutated normal cells can be become cancerous.
From the laboratory of Dominic Esposito, PhD, National Cancer Institute/NIH.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
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| Product Type: | Plasmid |
| Name: | KRAS4b Baculovirus Expression Vectors |
| Gene/insert name: | Human KRAS4b |
| Accession ID: | P01116 |
| Organism: | Human |
| Antibiotic Resistance: | Ampicillin/Gentamycin |
| Fusion Tag(s): | Aminoterminal His6-MBP-tev |
| Format: | Liquid (10 mM Tris-Cl, pH 8.0, 0.1 mM EDTA) |
| Grow in E. coli at 37 C: | Yes |
| Selectable markers: | Ampicillin/Gentamycin |
| Cloning Site 5': | Gateway attB1 |
| Cloning Site 3': | Gateway attB2 |
| Insert Size: | 570 bp |
| Vector Backbone and Size: | pDest-636, 6050 bp |
| High or low copy: | High |
| Comments: | To create baculovirus, must be transformed into a DH10Bac or DE35 type strain |
| Storage: | -20C |
| Shipped: | Room Temperature as a liquid |
- Gillette WK, Esposito D, Abreu Blanco M, Alexander P, Bindu L, Bittner C, Chertov O, Frank PH, Grose C, Jones JE, Meng Z, Perkins S, Van Q, Ghirlando R, Fivash M, Nissley DV, McCormick F, Holderfield M, Stephen AG. Farnesylated and methylated KRAS4b: high yield production of protein suitable for biophysical studies of prenylated protein-lipid interactions. Sci Rep. 2015 Nov 2;5:15916. doi: 10.1038/srep15916. PubMed PMID: 26522388; PubMed Central PMCID: PMC4629113.
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