P2Y Receptor Expressing Human Astrocytoma Cell Lines
1321N1 human astrocytoma cell lines expressing various P2Y purinergic G protein-coupled receptors.
Highlights:
- Available expressing one of the following: P2Y1, P2Y4, P2Y11, P2Y12
- Distinguished from most other cultured cell lines in that native 1321N1 cells have no response to extracellular nucleotides
- Most other cell lines express one or more P2Y receptors, making them problematic for screening or assessing receptor function
Astrocytomas are a type of cancer of the brain. They originate in a particular kind of glial cells, star-shaped brain cells in the cerebrum called astrocytes. This type of tumor does not usually spread outside the brain and spinal cord and it does not usually affect other organs. P2Y receptors are a family of purinergic G protein coupled receptors that are stimulated by nucleotides.
From the laboratory of Robert A. Nicholas, PhD, University of North Carolina at Chapel Hill.
1321N1 human astrocytoma cell lines expressing various P2Y purinergic G protein-coupled receptors.
Highlights:
- Available expressing one of the following: P2Y1, P2Y4, P2Y11, P2Y12
- Distinguished from most other cultured cell lines in that native 1321N1 cells have no response to extracellular nucleotides
- Most other cell lines express one or more P2Y receptors, making them problematic for screening or assessing receptor function
Astrocytomas are a type of cancer of the brain. They originate in a particular kind of glial cells, star-shaped brain cells in the cerebrum called astrocytes. This type of tumor does not usually spread outside the brain and spinal cord and it does not usually affect other organs. P2Y receptors are a family of purinergic G protein coupled receptors that are stimulated by nucleotides.
From the laboratory of Robert A. Nicholas, PhD, University of North Carolina at Chapel Hill.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
Specifications
| Product Type: | Cell Line |
| Name: | See different names (receptors) above |
| Cell Type: | Glioblastoma |
| Accession ID: | P47900 |
| Organism: | Human |
| Source: | Glial Cell |
| Morphology: | Adherent cells |
| Biosafety Level: | BSL 2 |
| Subculturing: | Split sub-confluent cultures (90%) 1:6 to 1:10 using trypsin/EDTA |
| Growth Conditions: | Grown in 5% CO2 in high-glucose Dulbecco's modified Eagle's medium supplemented with 5% fetal bovine serum, 100 U/ml penicillin, and 0.1 mg/ml streptomycin. |
| Cryopreservation: | 10% DMSO and 90% FBS |
| Comments: | Slower growing cells, adherent |
| Storage: | LN2 |
| Shipped: | Dry Ice |
Provider
From the laboratory of Robert A. Nicholas, PhD, University of North Carolina at Chapel Hill.
Comments
Receptor Information:
- The P2Y1 receptor couples to Gq and is important in ADP-mediated platelet aggregation and activated by ADP
- The P2Y4 receptor is coupled to Gq and activated by UTP; the human receptor is antagonized by ATP
- The P2Y11 receptor is activated by ATP and couples to Gq and Gs, although the coupling to Gq occurs at lower concentration of agonist
- The P2Y12 receptor is important in platelet aggregation and is the target of clopidogrel and other platelet inhibitors; The receptor coupels to Gi
References
- Filtz, T, Q Li, JL Boyer, RA Nicholas, and TK Harden (1994) Cloning, expression, and pharmacological characterization of a P2Y-purinergic receptor from turkey brain. Mol Pharm, 46:8-14.
- Schachter, J, Li, Q, Boyer, J, Nicholas, RA, and Harden, TK (1996) Second messenger cascade specificity and pharmacological selectivity of the human P2Y1-purinoceptor. Brit J Pharmacol, 118:167-173.
- Nicholas, RA, Watt, WC, Lazarowski, ER, Li, Q, and Harden, TK (1996) The uridine nucleotide selectivity of three phospholipase C-activating P2 receptors: Identification of a UDP-selective, a UTP-selective, and an adenine and uridine triphosphate-specific receptor. Mol Pharmacol, 50:224-229.
- Kennedy, C, Qi, A-D, Herold, CL, Harden, TK and Nicholas, RA (2000) ATP, an agonist at the rat P2Y4 receptor, is an anatagonist at the human P2Y4 receptor. Mol Pharmacol 57:926-931.
- Qi, A-D, Kennedy, C, Harden, TK and Nicholas, RA (2001) Differential coupling of the human P2Y11 receptor to phospholipase C and adenylyl cyclase. Brit J Pharmacol 132: 318-326.
- Chhatriwala M, Ravi RG, Patel RI, Boyer JL, Jacobson KA, Harden TK. Induction of novel agonist selectivity for the ADP-activated P2Y1 receptor versus the ADP-activated P2Y12 and P2Y13 receptors by conformational constraint of an ADP analog. J Pharmacol Exp Ther. 2004 Dec;311(3):1038-43.
- Lazarowski ER, Harden TK. UDP-Sugars as Extracellular Signaling Molecules: Cellular and Physiologic Consequences of P2Y14 Receptor Activation. Mol Pharmacol. 2015 Jul;88(1):51-60.
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