6dEB Plasmids
These plasmids (pBP144 and pBP130, used together) allow for the production of 6-deoxyerythronolide B (6dEB), the polyketide precursor to erythromycin, when residing in a strain of E. coli (such as BAP1) capable of supporting heterologous polyketide production.
From the laboratory of Blaine A. Pfeifer, PhD, University at Buffalo.
These plasmids (pBP144 and pBP130, used together) allow for the production of 6-deoxyerythronolide B (6dEB), the polyketide precursor to erythromycin, when residing in a strain of E. coli (such as BAP1) capable of supporting heterologous polyketide production.
From the laboratory of Blaine A. Pfeifer, PhD, University at Buffalo.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
Specifications
| Product Type: | Plasmid |
| Gene/insert name: | pBP130, pBP144 |
| Antibiotic Resistance: |
pbBP130: Ampicillin pbBP144: Kanamycin |
| Fusion Tag(s): |
pbBP130: DEBS2, DEBS3 pbBP144: PCC, DEBS1 |
| Grow in E. coli at 37 C: | Yes |
| Selectable markers: |
pbBP130: Ampicillin pbBP144: Kanamycin |
| Vector Backbone and Size: |
pbBP130: pET21c, 5443 bp pbBP144: pET28a, 5369 bp |
| High or low copy: | Medium |
| Comments: | Please see the reference below and associated supplementary information for cloning details and generalized plasmid maps. |
| Storage: | -20C |
| Shipped: | Room Temperature |
Provider
From the laboratory of Blaine A. Pfeifer, PhD, University at Buffalo.
References
- Pfeifer BA, Admiraal SJ, Gramajo H, Cane DE, Khosla C. Biosynthesis of complex polyketides in a metabolically engineered strain of E. coli. Science. 2001; 291(5509):1790-2.
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