Zebrafish Novel Immune-Type Receptor 9 (Nitr9) Antibodies
These IgG2, k light chain mouse monoclonal antibodies were raised against protein and recognize zebrafish novel immune-type receptor 9 (Nitr9).
Highlights:
- Reacts with zebrafish novel immune-type receptor 9 (Nitr9)
- Suitable for Immunofluorescence, Western Blot and Flow Cytometry applications
The novel immune-type receptor (NITR) family includes activating and inhibitory forms that are predicted to function as natural killer receptors in teleost species. The only activating NITR gene in zebrafish is nitr9. The zebrafish nitr9 gene yields three alternatively spliced transcripts that encode three proteins isoforms: Nitr9-long (Nitr9L), Nitr9-short (Nitr9S), and Nitr9-supershort (Nitr9SS). Nitr9 transcripts are detectable in immune tissues, but are present at significantly higher levels in lymphocytes than in myeloid cells. The Nitr9L protein has been shown to physically partner with and signals via the adaptor protein Dap12.
From the laboratory of Jeffrey A. Yoder, PhD, North Carolina State University.
These IgG2, k light chain mouse monoclonal antibodies were raised against protein and recognize zebrafish novel immune-type receptor 9 (Nitr9).
Highlights:
- Reacts with zebrafish novel immune-type receptor 9 (Nitr9)
- Suitable for Immunofluorescence, Western Blot and Flow Cytometry applications
The novel immune-type receptor (NITR) family includes activating and inhibitory forms that are predicted to function as natural killer receptors in teleost species. The only activating NITR gene in zebrafish is nitr9. The zebrafish nitr9 gene yields three alternatively spliced transcripts that encode three proteins isoforms: Nitr9-long (Nitr9L), Nitr9-short (Nitr9S), and Nitr9-supershort (Nitr9SS). Nitr9 transcripts are detectable in immune tissues, but are present at significantly higher levels in lymphocytes than in myeloid cells. The Nitr9L protein has been shown to physically partner with and signals via the adaptor protein Dap12.
From the laboratory of Jeffrey A. Yoder, PhD, North Carolina State University.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Antibody |
| Antigen: | Zebrafish novel immune-type receptor 9 (Nitr9) |
| Accession ID: | Q5YCY6, AY570237.1, AY570238.1, EF690658.1 |
| Molecular Weight: | 34 kDa (Nitr9L), 30 kDa (Nitr9S) and 20 kDa (Nitr9SS). Nitr9L and Nitr9S may appear larger in western analyses due to glycosylation |
| Isotype: |
19.1.1: IgG2a, k light chain 90.10.5: IgG2b, k light chain |
| Clonality: | Monoclonal |
| Clone Name: |
19.1.1 90.10.5 |
| Reactivity: | Zebrafish |
| Immunogen: | Protein |
| Species Immunized: | Mouse |
| Epitope: | I-type Immunoglobulin domain (encoded by nucleotides 298-623 of GenBank NM 001005576.1) |
| Purification Method: | Protein G |
| Buffer: | PBS, 0.05% (w/v) Sodium Azide |
| Tested Applications: |
19.1.1: IF and FC with transfected cells (may not recognize Nitr9S isoform by FC) 90.10.5: WB with endogenous tissue (1:1000), IF and FC with transfected cells (may not recognize Nitr9S isoform by FC) |
| Comments: | May not recognize Nitr9S isoform by FC |
| Storage: | -20C |
| Shipped: | Cold Packs |
- Shah RN, Rodriguez-Nunez I, Eason DD, Haire RN, Bertrand JY, Wittamer V, Traver D, Nordone SK, Litman GW, Yoder JA. 2012. Development and characterization of anti-Nitr9 antibodies. Adv Hematol.2012:596925. PMID: 2304955
- Muire PJ, Hanson LA, Wills R, Petrie-Hanson L. Differential gene expression following TLR stimulation in rag1-/- mutant zebrafish tissues and morphological descriptions of lymphocyte-like cell populations. PLoS One. 2017 Sep 14;12(9):e0184077. doi: 10.1371/journal.pone.0184077. eCollection 2017. PubMed PMID: 28910320
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