HT-22rho-0 Cell Lines
HT-22rho-0 is a murine hippocampal neuronal cell line that is highly sensitive to glutamate and is used to study glutamate-induced toxicity in neuronal cells. Neuronal cells derive their ATP almost exclusively through mitochondrial oxidative phosphorylation, which is disrupted in HT-22 cells devoid of mtDNA (HT-22 rho-0 cells).
Highlights:
- Useful in studies aimed at elucidating the role of the oxidative phosphorylation in neuronal function
From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.
HT-22rho-0 is a murine hippocampal neuronal cell line that is highly sensitive to glutamate and is used to study glutamate-induced toxicity in neuronal cells. Neuronal cells derive their ATP almost exclusively through mitochondrial oxidative phosphorylation, which is disrupted in HT-22 cells devoid of mtDNA (HT-22 rho-0 cells).
Highlights:
- Useful in studies aimed at elucidating the role of the oxidative phosphorylation in neuronal function
From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
Specifications
| Product Type: | Cell Line |
| Name: | HT-22 rho-0 or control |
| Cell Type: | Hippocampal neuronal cells |
| Morphology: | Epithelial |
| Organism: | Mouse |
| Source: | Brain/hippocampus |
| Growth Conditions: | DMEM high glucose, 10% FBS, 1 mM pyruvate, 50 ug/ml uridine |
| Subculturing: | Grows slow. Split 1:3 twice a week, or as needed. Change medium 1-2 times a week. Do not grow to confluence. |
| Cryopreservation: | Growth medium+10% DMSO |
| Comments: | Cells are sensitive to acidification of the medium. |
| Storage: | Liquid Nitrogen |
| Shipped: | Dry Ice |
Provider
From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.
References
- Khozhukhar N, Spadafora D, Rodriguez Y, Alexeyev M. Elimination of Mitochondrial DNA from Mammalian Cells. Curr Protoc Cell Biol. 2018;78(1):20.11.1-20.11.14.
- Davis J.B., Maher P. Protein kinase C activation inhibits glutamate-induced cytotoxicity in a neuronal cell line. Brain Res. 652:169-173(1994).
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