A549p0#1 Cell Lines
A549p0#1a is a human cell line derived from lung adenocarcinoma that lacks mitochondrial DNA, lack functional mitochondrial electron transport chain, and is auxotrophic for uridine and pyruvate.
Hightlights:
- Derived from A549 lung adenocarcinoma by treating cells with ethidium bromide
- Non-treated, control cell line also available
- Lacks mitochondrial DNA, lack functional mitochondrial electron transport chain, and is auxotrophic for uridine and pyruvate.
From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.
A549p0#1a is a human cell line derived from lung adenocarcinoma that lacks mitochondrial DNA, lack functional mitochondrial electron transport chain, and is auxotrophic for uridine and pyruvate.
Hightlights:
- Derived from A549 lung adenocarcinoma by treating cells with ethidium bromide
- Non-treated, control cell line also available
- Lacks mitochondrial DNA, lack functional mitochondrial electron transport chain, and is auxotrophic for uridine and pyruvate.
From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
Specifications
| Product Type: | Cell Line |
| Name: | A549p0#1a, A549p0#1a or control |
| Cell Type: | Epithelial |
| Morphology: | Epithelial |
| Organism: | Human |
| Source: | Lung |
| Growth Conditions: | DMEM high glucose, 10% FBS, 1 mM pyruvate, 50 ug/ml uridine |
| Subculturing: | Grows slow. Split 1:3 twice a week, or as needed. Change medium 1-2 times a week. Do not grow to confluence. |
| Cryopreservation: | Growth medium+10% DMSO |
| Comments: | Do not allow for medium to acidify as it may lead to cell death |
| Storage: | Liquid Nitrogen |
| Shipped: | Dry Ice |
Provider
From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.
References
- Khozhukhar N, Spadafora D, Rodriguez Y, Alexeyev M. Elimination of Mitochondrial DNA from Mammalian Cells. Curr Protoc Cell Biol. 2018;78(1):20.11.1-20.11.14.
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