Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17] Antibody Expression Plasmids
![pBG715, Anti-[mBG17] Heavy Chain Expression Plasmid](https://www.kerafast.com/MediaStorage/Product/Images/Large/2221_2001202001271922200.jpg "pBG715, Anti-[mBG17] Heavy Chain Expression Plasmid")
Plasmids expressing heavy and kappa chains, which when co-transfected into mammalian cells (such as 293F) will produce secreted antibody mBG17 that recognizes SARS-CoV-2 nucleocapsid antigen.
Highlights:
- Recognizes SARS-CoV2 Nucleocapsid protein
- mBG17 did show cross reaction with SARS-CoV nucleocapsid protein and minor cross-reaction with HuCoV-NL63 nucleocapsid protein was observed by ELISA, but no cross-reaction was observed with other coronavirus N proteins.
- Suitable for ELISA, immunofluorescence assay, and western blot applications
A panel of mouse monoclonal antibodies targeting the SARS-CoV-2 N protein were generated and characterized. Truncated recombinant N protein was expressed and purified, used the recombinant antigen to immunize mice and generated a panel of hybridomas, and tested the resulting clones for activity in western blots, ELISAs, and immunofluorescence assays with SARS-CoV-2 infected cells. Cross-reactivity of the antibodies against SARS-CoV, MERS-CoV, HuCoV-NL63, HuCoV-HKU1, HuCoV-OC43and HuCoV-229E N protein was tested.
From the laboratory of Brian J. Geiss, PhD, Colorado State University.
Plasmids expressing heavy and kappa chains, which when co-transfected into mammalian cells (such as 293F) will produce secreted antibody mBG17 that recognizes SARS-CoV-2 nucleocapsid antigen.
Highlights:
- Recognizes SARS-CoV2 Nucleocapsid protein
- mBG17 did show cross reaction with SARS-CoV nucleocapsid protein and minor cross-reaction with HuCoV-NL63 nucleocapsid protein was observed by ELISA, but no cross-reaction was observed with other coronavirus N proteins.
- Suitable for ELISA, immunofluorescence assay, and western blot applications
A panel of mouse monoclonal antibodies targeting the SARS-CoV-2 N protein were generated and characterized. Truncated recombinant N protein was expressed and purified, used the recombinant antigen to immunize mice and generated a panel of hybridomas, and tested the resulting clones for activity in western blots, ELISAs, and immunofluorescence assays with SARS-CoV-2 infected cells. Cross-reactivity of the antibodies against SARS-CoV, MERS-CoV, HuCoV-NL63, HuCoV-HKU1, HuCoV-OC43and HuCoV-229E N protein was tested.
From the laboratory of Brian J. Geiss, PhD, Colorado State University.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Plasmid |
| Gene/insert name: | pBG715, Anti-[mBG17] Heavy Chain Expression Plasmid |
| Organism: | E.coli |
| Antibiotic Resistance: | Kanomycin |
| Grow in E. coli at 37 C: | Yes |
| Cloning Site 5': | SacI |
| Cloning Site 3': | NotI |
| Insert Size: | 1389 bp (Heavy) 732 bp (Kappa) |
| Vector Backbone and Size: | pCMV (3952 bp) |
| High or low copy: | High |
| Storage: | -20C |
| Shipped: | Room temperature |
1. James S. Terry, Loran BR. Anderson, Michael S. Scherman, Carley E. McAlister, Rushika Perera, Tony Schountz, Brian J. Geiss. Development of a SARS-CoV-2 nucleocapsid specific monoclonal antibody. Virology. 2020 Sep 3:2020;09.03.280370. View Article
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