Human TNF alpha, RTNF001
Recombinant human TNF alpha protein, produced from E. coli.
Tumor necrosis factor-α (TNF-α) also known as cachectin, is a pleiotropic cytokine secreted mainly by monocytes and macrophages. It is a trimetric protein encoded within the major histocompatibility complex. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-a converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-a plays a major role in antitumor activity, inflammation, immune system development, apoptosis, anorexia, cachexia, septic shock, viral replication and lipid metabolism. TNF-a also shows antiviral effects against both DNA and RNA Viruses and it induces production of several other cytokines.
Recombinant human TNF alpha protein, produced from E. coli.
Tumor necrosis factor-α (TNF-α) also known as cachectin, is a pleiotropic cytokine secreted mainly by monocytes and macrophages. It is a trimetric protein encoded within the major histocompatibility complex. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-a converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-a plays a major role in antitumor activity, inflammation, immune system development, apoptosis, anorexia, cachexia, septic shock, viral replication and lipid metabolism. TNF-a also shows antiviral effects against both DNA and RNA Viruses and it induces production of several other cytokines.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Protein |
| Name: | Human TNF alpha |
| Alternative Name(s): | cachectin |
| Accession ID: | P01375 |
| Source: | Human Ð expressed in E. coli |
| Molecular Weight: | 17.4 kDa |
| Amino Acid Sequence: | 77-VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIA VSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEK GDRLSAEINRPDYLDFAESGQVYFGIIAL-233 |
| Fusion Tag(s): | C-terminus 6 _ His tag |
| Format: | Lyophilized; It is recommended to briefly spin the vial prior to opening to bring the contents to the bottom. Reconstitute lyophilized protein in buffer (10 mM Tris-HCl, pH 8.5 + 0.1% Glucose) |
| Purity: | >96 %, SDS-PAGE (Coomassie blue staining) |
| Buffer: | 10 mM Tris-HCl + 0.1% Glucose |
| Activity: | ED50 in the range of 100 pg/mL as determined by the dose dependent cytotoxic effect on murine L929 cells in the presence of Actinomycin D and potency in the range of 0.01-0.05 pg/mL |
| Comments: | Manufactured in an ISO 9001:20015 Certified Laboratory |
| Storage: | Store lyophilized human TNF-a at +2C to +8 C, preferably desiccated. Upon reconstitution, prepare working aliquots and store at -20C. Avoid repeated freeze/thaw cycles. |
| Shipped: | Cold packs |
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