Paul Knox, PhD, University of Leeds

The Knox laboratory at the Centre for Plant Sciences, University of Leeds is focused around plant cell walls. Current interests include: generation and use of molecular probes including monoclonal antibodies to dissect cell wall structures and functions, primary cell walls in cell expansion and cell adhesion; structure and function of polysaccharides secreted by land plants.

PlantProbes

The Knox laboratory has been sharing their plant cell wall antibodies through their PlantProbes distribution service. The lab partnered with Kerafast to ensure and extend antibody availability to scientists worldwide.

Products

References

  1. Smallwood, M., Martin, H. & Knox, J.P. An epitope of rice threonine- and hydroxyproline-rich glycoprotein is common to cell wall and hydrophobic plasma-membrane glycoproteins. Planta (1995) 196: 510.
  2. Smallwood M, Yates EA, Willats WGT, Martin H, Knox JP (1996) Immunochemical comparison of membrane-associated and secreted arabinogalactan-proteins in rice and carrot. Planta 198, 452-459
  3. Yates EA, Valdor JF, Haslam SM, Morris HR, Dell A, Mackie W, Knox JP. Characterization of carbohydrate structural features recognized by anti-arabinogalactan-protein monoclonal antibodies. Glycobiology. 1996 Mar;6(2):131-9. PubMed PMID: 8727785.
  4. Jones L, Seymour GB, Knox JP. Localization of Pectic Galactan in Tomato Cell Walls Using a Monoclonal Antibody Specific to (1[->]4)-[beta]-D-Galactan. Plant Physiol. 1997 Apr;113(4):1405-1412.
  5. Willats WG, Orfila C, Limberg G, Buchholt HC, van Alebeek GJ, Voragen AG, Marcus SE, Christensen TM, Mikkelsen JD, Murray BS, Knox JP. Modulation of the degree and pattern of methyl-esterification of pectic homogalacturonan in plant cell walls. Implications for pectin methyl esterase action, matrix properties, and cell adhesion. J Biol Chem. 2001 Jun 1;276(22):19404-13.
  6. Clausen MH, Willats WG, Knox JP. Synthetic methyl hexagalacturonate hapten inhibitors of anti-homogalacturonan monoclonal antibodies LM7, JIM5 and JIM7. Carbohydr Res. 2003 Aug 12;338(17):1797-800.
  7. Moller I, Marcus SE, Haeger A, et al. High-throughput screening of monoclonal antibodies against plant cell wall glycans by hierarchical clustering of their carbohydrate microarray binding profiles. Glycoconjugate Journal. 2008;25(1):37-48.
  8. Verhertbruggen Y, Marcus SE, Haeger A, Verhoef R, Schols HA, McCleary BV, McKee L, Gilbert HJ, Knox JP. Developmental complexity of arabinan polysaccharides and their processing in plant cell walls. Plant J. 2009 Aug;59(3):413-25.
  9. Verhertbruggen Y, Marcus SE, Haeger A, Ordaz-Ortiz JJ, Knox JP. An extended set of monoclonal antibodies to pectic homogalacturonan. Carbohydr Res. 2009 Sep 28;344(14):1858-62.
  10. Pedersen HL, Fangel JU, McCleary B, Ruzanski C, Rydahl MG, Ralet MC, Farkas V, von Schantz L, Marcus SE, Andersen MC, Field R, Ohlin M, Knox JP, Clausen MH, Willats WG. Versatile high resolution oligosaccharide microarrays for plant glycobiology and cell wall research. J Biol Chem. 2012 Nov 16;287(47):39429-38.
  11. Andersen MC, Boos I, Marcus SE, Kraun SK, Rydahl MG, Willats WG, Knox JP, Clausen MH. Characterization of the LM5 pectic galactan epitope with synthetic analogues of Î?-1,4-d-galactotetraose. Carbohydr Res. 2016 Dec 21;436:36-40.
  12. Cornuault V, Buffetto F, Marcus SE, Crépeau M-J, Guillon F, Ralet M-C, Knox P (2017) LM6-M: a high avidity rat monoclonal antibody to pectic α-1,5-L-arabinan. BioRÏ?iv https://doi.org/10.1101/161604
  13. Ruprecht C, Bartetzko MP, Senf D, Dallabernardina P, Boos I, Andersen MCF, Kotake T, Knox JP, Hahn MG, Clausen MH, Pfrengle F (2017) A synthetic glycan microarray enables epitope mapping of plant cell wall glycan-directed antibodies. Plant Physiology https://doi.org/10.1104/pp.17.00737
  14. Cornuault V, Buffetto F, Rydahl MG, et al. Monoclonal antibodies indicate low-abundance links between heteroxylan and other glycans of plant cell walls. Planta. 2015;242(6):1321‐1334.
  15. Torode TA, O'Neill R, Marcus SE, et al. Branched Pectic Galactan in Phloem-Sieve-Element Cell Walls: Implications for Cell Mechanics. Plant Physiol. 2018;176(2):1547-1558.
  16. Marcus SE, Blake AW, Benians TA, et al. Restricted access of proteins to mannan polysaccharides in intact plant cell walls. Plant J. 2010;64(2):191-203.
  17. Manabe Y, Nafisi M, Verhertbruggen Y, et al. Loss-of-function mutation of REDUCED WALL ACETYLATION2 in Arabidopsis leads to reduced cell wall acetylation and increased resistance to Botrytis cinerea. Plant Physiol. 2011;155(3):1068-1078.
  18. Pedersen HL, Fangel JU, McCleary B, et al. Versatile high resolution oligosaccharide microarrays for plant glycobiology and cell wall research. J Biol Chem. 2012;287(47):39429-39438.
  19. Knox JP, Linstead PJ, Cooper JPC, Roberts K. Developmentally regulated epitopes of cell surface arabinogalactan proteins and their relation to root tissue pattern formation. Plant J. 1991;1(3):317-326.
  20. Moller I, Marcus SE, Haeger A, et al. High-throughput screening of monoclonal antibodies against plant cell wall glycans by hierarchical clustering of their carbohydrate microarray binding profiles. Glycoconj J. 2008;25(1):37-48.
  21. Wilkinson et al. (2017) J. Cereal Science 74, 155-164.
  22. Smallwood M, Yates EA, Willats WGT, Martin H, Knox JP (1996) Immunochemical comparison of membrane-associated and secreted arabinogalactan-proteins in rice and carrot. Planta 198, 452-459
  23. Wilkinson et al. (2017) J. Cereal Science 74, 155-164.
  24. Stacey NJ, Roberts K, Knox JP. Patterns of expression of the JIM4 arabinogalactan-protein epitope in cell cultures and during somatic embryogenesis in Daucus carota L. Planta. 1990;180(2):285‐292.
  25. Yates EA, Valdor JF, Haslam SM, et al. Characterization of carbohydrate structural features recognized by anti-arabinogalactan-protein monoclonal antibodies. Glycobiology. 1996;6(2):131‐139.
  26. Pennell RI, Janniche L, Kjellbom P, Scofield GN, Peart JM, Roberts K. Developmental Regulation of a Plasma Membrane Arabinogalactan Protein Epitope in Oilseed Rape Flowers. Plant Cell. 1991;3(12):1317‐1326.
  27. Knox JP, Linstead PJ, Cooper JPC, Roberts K. Developmentally regulated epitopes of cell surface arabinogalactan proteins and their relation to root tissue pattern formation. Plant J. 1991;1(3):317‐326.
  28. Moller I, Marcus SE, Haeger A, et al. High-throughput screening of monoclonal antibodies against plant cell wall glycans by hierarchical clustering of their carbohydrate microarray binding profiles. Glycoconj J. 2008;25(1):37‐48.