DMT1 Expressing HEK293T Cell Line
Immortalized cell line HEK293 that stably expresses mouse divalent metal transporter-1 (DMT1).
Highlights:
- Ideal tool to screen for potential iron transport inhibitors
- Characterized to take up more ferrous iron at lower pH
- DMT1 is found both on the cell surface and in intracellular vesicles
The divalent metal transporter 1 (DMT1), also known as natural resistance-associated macrophage protein 2 (NRAMP 2), and divalent cation transporter 1 (DCT1), binds a variety of divalent metals, but is best known for its role in transporting ferrous iron (Fe2+); DMT1 expression is regulated by body iron stores to maintain iron homeostasis. In the digestive tract, it is located on the apical membrane of enterocytes, where it carries out H+ coupled transport of divalent metal cations from the intestinal lumen into the cell. DMT1 is also involved in the delivery of iron to peripheral tissues by transferrin. Defects in the DMT1 gene cause microcytic anemia in the mk mouse, an animal model that displays defective dietary iron absorption.
From the laboratory of Marianne Wessling-Resnick, PhD, Harvard University.
Immortalized cell line HEK293 that stably expresses mouse divalent metal transporter-1 (DMT1).
Highlights:
- Ideal tool to screen for potential iron transport inhibitors
- Characterized to take up more ferrous iron at lower pH
- DMT1 is found both on the cell surface and in intracellular vesicles
The divalent metal transporter 1 (DMT1), also known as natural resistance-associated macrophage protein 2 (NRAMP 2), and divalent cation transporter 1 (DCT1), binds a variety of divalent metals, but is best known for its role in transporting ferrous iron (Fe2+); DMT1 expression is regulated by body iron stores to maintain iron homeostasis. In the digestive tract, it is located on the apical membrane of enterocytes, where it carries out H+ coupled transport of divalent metal cations from the intestinal lumen into the cell. DMT1 is also involved in the delivery of iron to peripheral tissues by transferrin. Defects in the DMT1 gene cause microcytic anemia in the mk mouse, an animal model that displays defective dietary iron absorption.
From the laboratory of Marianne Wessling-Resnick, PhD, Harvard University.
| Product Type: | Cell Line |
| Name: | HEK293T(DMT1) |
| Cell Type: | HEK293T (human embryonic kidney expressing T antigen) |
| Accession ID: | P49281, CVCL_HA71 |
| Morphology: | Fibroblast |
| Source: | Human embryonic kidney |
| Organism: | Human |
| Biosafety Level: | BSL2 |
| Subculturing: | Selected by puromycin (do not need to maintain presence of antibiotic) |
| Growth Conditions: | Minimal essential medium (aMEM) supplemented with 50 U/ml penicillin, 50 mg/ml streptomycin, and 10% fetal bovine serum |
| Cryopreservation: | Growth Medium + 10% DMSO |
| Storage: | Liquid nitrogen |
| Shipped: | Dry ice |
- Wetli HA, Buckett PD, Wessling-Resnick M. Small-molecule screening identifies the selanazal drug ebselen as a potent inhibitor of DMT1-mediated iron uptake. Chem Biol. 2006 Sep;13(9):965-72.
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