Immortalized Human Mast Cell Line (LUVA) that is easily cultured without growth factors.
Highlights:
Mast cells exist in several types of tissues and play a role in allergy and anaphylaxis as a factor in inflammation response. They are also involved in wound healing and defense against pathogens. These cells express the high-affinity receptors that are specific to IgE, FceRI, on their surface, making IgE molecules cell-surface receptors for an antigen. When bound IgE molecules find a substrate, mast cells release inflammatory mediators like histamine.
From the laboratory of John W. Steinke, PhD, Larry C. Borish, MD, and S. Brandon Early, University of Virginia.
Part of The Investigator's Annexe program.
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Product Type: | Cell Line |
Name: | Human Mast Cell Line (LUVA) |
Cell Type: | Mast cell |
Accession ID: | CVCL_5G48 |
Source: | Blood CD34+ derived |
Organism: | Human |
Morphology: | Suspension and adherent |
Biosafety Level: | BSL1 |
Subculturing: | Cells can be split 1:2 – 1:20. Cells split 1:10 will be confluent in 3-4 days. |
Growth Conditions: | StemPro-34 SFM (500mL), L-glutamine (2mM final), 5mL Pen Strep (10,000 U/mL), 1mL Primocin (50mg). See LUVA Protocol for instructions. |
Cryopreservation: | 90% Complete media, 10% DMSO (See LUVA Protocol for instructions.) |
Comments: | The Steinke lab has noted a difference in how the cells are behaving that is different from the original publication. In the article, LUVA cells had high levels of expression of FceRI, however levels of around 5% by cell surface staining are currently being observed by the provider. Higher levels with intracellular staining are seen and very high mRNA expression for FceRI is detected. The reason for this difference is not clear. |
Storage: | Liquid nitrogen |
Shipped: | Dry ice |
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