pGLuc-TATA KBS/ZBE Plasmids
These plasmids were constructed using pGLuc-basic Gaussia luciferase plasmid as the backbone and contain four copies of the consensus Kaiso Binding Site (4xKBS) or Znf131 DNA Binding Element (4xZBE).
Highlights:
- A TATA sequence was inserted into the BamHI site in the MCS
- The BamHI site is reconstituted at the 5’ end of the TATA sequence and an NdeI site was inserted as a diagnostic marker restriction site
Zinc finger protein, 131 plays a role during development and organogenesis as well as in the function of the adult central nervous system. In addition, it has been shown to be involved in transcriptional regulation as a repressor of ESR1/ER-alpha signaling.
Kaiso, a transcription factor was first identified as a specific binding partner for the catenin p120ctn, which is aberrantly expressed or absent in human breast, colon and skin cancers. Kaiso is also a member of the POZ-zinc finger family of transcription factors implicated as oncoproteins or tumor suppressors, and currently it is the only known POZ protein with bi-modal DNA-binding and transcriptional repression activity.
From the laboratory of Juliet M. Daniel, PhD, McMaster University
Part of The Investigator's Annexe program.
These plasmids were constructed using pGLuc-basic Gaussia luciferase plasmid as the backbone and contain four copies of the consensus Kaiso Binding Site (4xKBS) or Znf131 DNA Binding Element (4xZBE).
Highlights:
- A TATA sequence was inserted into the BamHI site in the MCS
- The BamHI site is reconstituted at the 5’ end of the TATA sequence and an NdeI site was inserted as a diagnostic marker restriction site
Zinc finger protein, 131 plays a role during development and organogenesis as well as in the function of the adult central nervous system. In addition, it has been shown to be involved in transcriptional regulation as a repressor of ESR1/ER-alpha signaling.
Kaiso, a transcription factor was first identified as a specific binding partner for the catenin p120ctn, which is aberrantly expressed or absent in human breast, colon and skin cancers. Kaiso is also a member of the POZ-zinc finger family of transcription factors implicated as oncoproteins or tumor suppressors, and currently it is the only known POZ protein with bi-modal DNA-binding and transcriptional repression activity.
From the laboratory of Juliet M. Daniel, PhD, McMaster University
Part of The Investigator's Annexe program.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Plasmid |
| Gene/insert name: | 4xKBS |
| Antibiotic Resistance: | Amp |
| Grow in E. coli at 37 C: | Yes |
| Selectable markers: | NeoR |
| Cloning Site 5': | EcoRI |
| Cloning Site 3': | SacI |
| Insert Size: | 87 bp |
| Vector Backbone and Size: | pGLuc, 4971 bp |
| Comments: | A TATA sequence was inserted into the BamHI site in the MCS. Also contains 4 copies of Kaiso Binding Site (KBS) |
| Storage: | Up to 1 month at 4C. Long-term, -20C |
| Shipped: | Ambient temperature, spotted on filter paper |
pGLuc-TATA KBS/ZBE Plasmids
| Gene/Insert Name: | 4xKBS | 4xZBE |
| Description: | 4 copies of Kaiso Binding Site (KBS) | 4 copies of Znf131 DNA Binding Element (ZBE) |
| Insert Size (bp): | 87 | |
| Species: | Human | Human |
| Selective Markers: | NeoR | NeoR |
| Vector Backbone and Size: | pGLuc, 4971 bp | pGLuc, 4971 bp |
| Cloning Site 5': | EcoRI | EcoRI |
| Cloning Site 3': | SacI | SacI |
| Antibiotic Resistance: | Amp | Amp |
| Standard E. coli @ 37C?: | Yes | Yes |
| Comments: | A TATA sequence was inserted into the BamHI site in the MCS | |
| Storage Temperature: | Up to 1 month at 4C. Long-term, -20C | |
| Shipped: | Room temperature, spotted on filter paper | |
- Daniel JM, Spring CM, Reynolds AB, Crawford HC and Baig A. The p120ctn-binding partner Kaiso is a bi-modal DNA-binding protein that recognizes both a sequence-specific consensus and methylated CpG dinucleotides. (2002) Nucleic Acids Res. 30: 2911-2919.
- Kelly KF, Spring CM, Otchere AA and Daniel JM. NLS-dependent nuclear localization of p120ctn is necessary to relieve Kaiso-mediated transcriptional repression. (2004) J. Cell Sci. 117: 2675-2686
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