Anti-Complement Activated C3 [I3/15] Antibody
![Anti-Complement Activated C3 [I3/15] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/1217_2001202001263912160.jpg "Anti-Complement Activated C3 [I3/15] Antibody")
This mouse IgG1k monoclonal antibody was generated against purified human complement C3dg and recognizes human and baboon complement C3 fragments C3b, iC3b, and C3dg.
Highlights:
- Reacts with human and baboon C3b, iC3b, C3dg
- Useful as capture antibody in activated C3 ELISA
- Suitable for Western Blot and ELISA applications
The complement system consists of a number of small proteins found in the blood, generally synthesized by the liver, and normally circulating as inactive precursors (pro-proteins). When stimulated by one of several triggers, proteases in the system cleave specific proteins to release cytokines and initiate an amplifying cascade of further cleavages. The end-result of this activation cascade is massive amplification of the response and activation of the cell-killing membrane attack complex.
From the laboratory of Martin Oppermann, MD, University Medical Center Göttingen.
This mouse IgG1k monoclonal antibody was generated against purified human complement C3dg and recognizes human and baboon complement C3 fragments C3b, iC3b, and C3dg.
Highlights:
- Reacts with human and baboon C3b, iC3b, C3dg
- Useful as capture antibody in activated C3 ELISA
- Suitable for Western Blot and ELISA applications
The complement system consists of a number of small proteins found in the blood, generally synthesized by the liver, and normally circulating as inactive precursors (pro-proteins). When stimulated by one of several triggers, proteases in the system cleave specific proteins to release cytokines and initiate an amplifying cascade of further cleavages. The end-result of this activation cascade is massive amplification of the response and activation of the cell-killing membrane attack complex.
From the laboratory of Martin Oppermann, MD, University Medical Center Göttingen.
| Product Type: | Antibody |
| Antigen: | Human Complement C3-Fragments C3b/iC3b/C3dg |
| Isotype: | IgG1k |
| Clonality: | Monoclonal |
| Clone Name: | I3-15 |
| Reactivity: | Human, baboon |
| Immunogen: | Purified human C3dg |
| Species Immunized: | Mouse (Balb/c) |
| Epitope: | Activation-dependent neo-Epitope on C3b/iC3b/C3dg, but not on C3 |
| Buffer: | PBS, 0.05% (w/v) Sodium Azide |
| Tested Applications: | WB and ELISA |
| Storage: | -20C |
| Shipped: | Cold packs |
| Clone | Reactivity | Isotype | Epitope | Applications | Functional Properties |
| M20/6 | Human Ba / Factor B | IgG1k | N/A | WB, ELISA, IP | Detecting Ab in Ba ELISA (together with D22/3) |
| D22/3 | Human Ba | IgG2b/k | Neo-Epitope on Ba (Glu215, Arg234) | WB, ELISA, Flow Cytometry | Capture Ab in Ba ELISA (together with M20/6) |
| I3/15 | Human and baboon activated C3 | IgG1k | Neo-Epitope on C3b/iC3b/C3dg | WB, ELISA | Capture Ab in actC3 ELISA (together with rab anti-C3dg) |
| Z22/8 | Porcine C3a/C3a(desArg) | IgG2b/k | Neo-Epitope on C3a/C3a(desArg) | WB, ELISA, IP | Capture Ab in C3a ELISA (together with K5/9); blocks C3a biological activity |
| K5/9 | Porcine C3a/C3a(desArg) | IgG1k | N/A | WB, ELISA | Capture Ab in C3a ELISA (together with Z22/8); blocks C3a biological activity |
| G25/2 | Human and baboon C5/C5a/C5a(desArg) | IgG1k | N/A | WB, ELISA, IP | Detecting Ab in C5a ELISA (together with C17/5); blocks C5a biological activity |
| C17/5 | Human and baboon C5/C5a/C5a(desArg) | IgG1k | Neo-Epitope on C5a/C5a(desArg) | WB, ELISA | Capture Ab in C5a ELISA (together with G25/2); blocks C5a biological activity |
| T13/9 | Porcine C5a/C5a(desArg) | IgG1k | Neo-Epitope on C5a (Tyr57- Gly74) | WB, ELISA, IP | Capture Ab in C5a ELISA (together with rab anti-C5a); blocks C5a biological activity |
- Oppermann, M., Baumgarten, H., Brandt, E., Gottsleben, W., Kurts, C., and Götze, O. (1990) J. Immunol. Methods 133, 181-190
- Oppermann, M., Kurts, C., Zierz, R., Quentin, E., Weber, M. H., and Götze, O. (1991) Kidney Int. 40, 939-947
- Mollnes et al. (1993) Clin Exp Immunol 91,295-300
- Scholl et al. (2008) PLoS ONE. 3(7):e2593
If you publish research with this product, please let us know so we can cite your paper.
